Synthesis of DNA -- Where the Energy Comes From                                                                         11-2

Reaction 1 --  Chain + TMP (or dCMP, etc.) -----> Longer chain:

 

Reaction 2 -- Hydrolysis of 2 ATP to ADP

 2 ATP + 2H2O --> 2 ADP + 2 Pi Delta Go = -14 kcal/mole


Reaction 3 -- Energy of ATP is used to convert TMP, (d)CMP,  etc. to TTP, (d) CTP etc.  as follows:

 2 ATP + TMP (or dCMP etc.) --> 2 ADP + TTP (or dCTP  etc.) Delta G� = 0 kcal/mole


Reaction 4 -- Chain + TTP (or dCTP etc.) ---> Longer Chain

Reaction 5: Hydrolysis of  P-Pi

P - Pi   + H2O

Pyrophosphatase
---------------------->

 2 Pi Delta G = -7 Kcal/mole

Overall Summary