An example restriction map (using a subset of all restriction enzymes)
Human interferon alpha gene, protein-coding sequence underlined
Enzyme No. Positions Recognition Enzyme No. Positions
Recognition
name cuts of sites sequence
name cuts of sites
sequence
A=AcsI 1 826
r/aatty E=EcoNI 3 413 910 1254
cctnn/nnnagg
B=BbsI 1
441 gaagac../ Hh=HhaI
1
3 gcg/c
BsD=BseDI 3 603 695 995 c/cnngg Hi=HindII
1 1040 gty/rac
BsR=BseRI 2 647 705 gaggag../ M=MaeIII
2 368 891 /gtnac
D=DdeI 3 46 456 550 c/tnag P=PvuII
3 317 554 852 cag/ctg
E=EarI 1 780
ctcttc../ S=SfaNI 2 497
628 gcatc../
Ha=HaeIII 2 514 538 gg/cc T=TspRI
2 206 838 /..cagtg ../
r= a or G y= t or c n = any base
/= cleavage position within sequence
For non-palindomic sequences, consider bottom strand as well
top.
HhaI
DdeI
gcgcctcttatgtacccacaaaaatctattttcaaaaaagttgctctaagaatatagttatcaagttaagtaaaa
base pairs
cgcggagaatacatgggtgtttttagataaaagttttttcaacgagattcttatatcaatagttcaattcatttt 1 to
75
tgtcaatagccttttaatttaatttttaattgttttatcattctttgcaataataaaacattaactttatacttt
base pairs
acagttatcggaaaattaaattaaaaattaacaaaatagtaagaaacgttattattttgtaattgaaatatgaaa 76
to 150
TspRI
ttaatttaatgtatagaatagagatatacataggatatgtaaatagatacacagtgtatatgtgattaaaatata
base pairs
aattaaattacatatcttatctctatatgtatcctatacatttatctatgtgtcacatatacactaattttatat 151
to 225
atgggagattcaatcagaaaaaagtttctaaaaaggctctggggtaaaagaggaaggaaacaataatgaaaaaaa
base pairs
taccctctaagttagtcttttttcaaagatttttccgagaccccattttctccttcctttgttattacttttttt 226
to 300
PvuII
MaeIII
tgtggtgagaaaaacagctgaaaacccatgtaaagagtgtataaagaaagcaaaaagagaagtagaaagtaacac
base pairs
acaccactctttttgtcgacttttgggtacatttctcacatatttctttcgtttttctcttcatctttcattgtg 301
to 375
EcoNI
BbsI
aggggcatttggaaaatgtaaacgagtatgttccctatttaaggctaggcacaaagcaaggtcttcagagaacct
base pairs
tccccgtaaaccttttacatttgctcatacaagggataaattccgatccgtgtttcgttccagaagtctcttgga
376 to 450
DdeI
SfaNI
HaeIII
ggagcctaaggtttaggctcacccatttcaaccagtctagcagcatctgcaacatctacaatggccttgaccttt
base pairs
cctcggattccaaatccgagtgggtaaagttggtcagatcgtcgtagacgttgtagatgttaccggaactggaaa 451
to 525
HaeIII DdeI PvuII
gctttactggtggccctcctggtgctcagctgcaagtcaagctgctctgtgggctgtgatctgcctcaaacccac
base pairs
cgaaatgaccaccgggaggaccacgagtcgacgttcagttcgacgagacacccgacactagacggagtttgggtg 526
to 600
BseDI
SfaNI
BseRI
agcctgggtagcaggaggaccttgatgctcctggcacagatgaggagaatctctcttttctcctgcttgaaggac
base pairs
tcggacccatcgtcctcctggaactacgaggaccgtgtctactcctcttagagagaaaagaggacgaacttcctg
601 to 675
BseDI BseRI
agacatgactttggatttccccaggaggagtttggcaaccagttccaaaaggctgaaaccatccctgtcctccat
base pairs
tctgtactgaaacctaaaggggtcctcctcaaaccgttggtcaaggttttccgactttggtagggacaggaggta 676
to 750
EarI
gagatgatccagcagatcttcaatctcttcagcacaaaggactcatctgctgcttgggatgagaccctcctagac
base pairs
ctctactaggtcgtctagaagttagagaagtcgtgtttcctgagtagacgacgaaccctactctgggaggatctg 751
to 825
AcsI TspRI
PvuII
MaeIII
aaattctacactgaactctaccagcagctgaatgacctggaagcctgtgtgatacagggggtgggggtgacagag
base pairs
tttaagatgtgacttgagatggtcgtcgacttactggaccttcggacacactatgtcccccacccccactgtctc
826 to 900
EcoNI
actcccctgatgaaggaggactccattctggctgtgaggaaatacttccaaagaatcactctctatctgaaagag
base pairs
tgaggggactacttcctcctgaggtaagaccgacactcctttatgaaggtttcttagtgagagatagactttctc 901
to 975
BseDI
HindII
aagaaatacagcccttgtgcctgggaggttgtcagagcagaaatcatgagatctttttctttgtcaacaaacttg
base pairs
ttctttatgtcgggaacacggaccctccaacagtctcgtctttagtactctagaaaaagaaacagttgtttgaac 976
to 1050
caagaaagtttaagaagtaaggaatgaaaactggttcaacatggaaatgattttcattgattcgtatgccagctc
base pairs
gttctttcaaattcttcattccttacttttgaccaagttgtacctttactaaaagtaactaagcatacggtcgag 1051
to 1125
acctttttatgatctgccatttcaaagactcatgtttctgctatgaccatgacacgatttaaatcttttcaaatg
base pairs
tggaaaaatactagacggtaaagtttctgagtacaaagacgatactggtactgtgctaaatttagaaaagtttac 1126
to 1200
EcoNI
ttttaggagtattaatcaacattgtattcagctcttaaggcactagtcccttacagaggaccatgctgactgat
base pairs
aaaaatcctcataattagttgtaacataagtcgagaattccgtgatcagggaatgtctcctggtacgactgacta 1201
to 1275
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Intro Bio 16-3