C2005/F2401 '09 -- Lecture 13 -- OUTLINE Last posted/updated 10/21/09 02:42 PM

I. RNA Synthesis vs DNA synthesis; see handout 12B

II. Sense & Anti-Sense; RNAi

III. Proof Reading (Editing) -- Why DNA polymerase and not RNA polymerase does this.

III. Translation -- How does "RNA make Protein?"

    A. Messenger RNA (mRNA)

• How is mRNA read?
• Using the Code Table

    B. Transfer RNA (tRNA)

• Why tRNA?
• Structure of tRNA
• How do the tRNA anticodons pair with the mRNA codons?

    C. How does the peptide chain grow?

    D. How do Ribosomes and ribosomal RNA (rRNA) fit in?

• Ribosomes -- Major Features -- How many sites for tRNA? A, P and E sites
• How Ribosomes Move
• Polyribosomes -- how many ribosomes per mRNA?
• Where is peptidyl transferase?

Any features of translation not covered will be wrapped up in the following class. Then we will consider (1) What happens if there are mistakes in transcription, translation or replication? (2) How does regulation of protein synthesis work in Prokaryotes?