C2005/F2401 '09 -- Outline For Lecture 17-- Restriction Enzymes & Genetic Engineering

Last Posted/Updated 11/10/09 03:45 PM

I. Viral Life Cycle & Viral Genetics

       A. Lytic Cycle

B. Lysogeny

C. Retroviruses

D. Viral Crosses; review of complementation vs. recombination

II. Restriction Enzymes (Restriction Endonucleases)

A. Discovery -- how they protect bacteria from viruses

B. Examples

C. Significance -- use for cloning, forensics, etc.
 

III. Probes -- Why you need them and how you get them.

IV. Blots -- Uses and Procedure.

Next time:  Molecular Cloning. How do you make a clone? How do you find the right one? And what do you find out from cloning eukaryotic genes? (Introns)