C2005/F2401 '10 -- Outline for Lecture #18

Posted/last updated 11/14/10 07:46 PM

I. Making a library -- how you actually do cloning

    A. Why bother?

    B. What's a library? How do you make one? (Handout 18A)

    C. What's a cloning vehicle, why do you need it, and what are its properties? (Handout 18B)

II. Finding the 'right' clone in a library -- Where is the one with the insert you want?

    A. What to look for

    B. Colony Hybridization (Handout 18B, cont.)

    C. How do you get a probe?

    D. What will you do with your clone?

III. What do you find if you clone a eukaryotic gene? (Handout 18C)

    A. Discovery  of Introns (Intervening DNA sequences)  

    B. Overview of Splicing

    C. Implications of splicing
 

Next time: Wrap up of splicing; then -- How does one eukaryotic cell make two?