In the problem book, question 3-1 B, and 3-3 C...re: competitive/non-c. inhibitors. How can I tell if the inhibitor is competitive or not?
The (apparent) Km measured in a plot of Vo vs. S in the presence of the inhibitor will be higher than in the absence of the inhibitor. A non-competitive inhibitor will have no effect on Km measurement. A noncompetitive inhibitor will decrease the Vmax of a reaction in a Vo vs, S experiment. A competitive inhibitor will not do this. The structure of a competitive inhibitor will resemble that of the substrate. A non-competitive inhibitor need have no such resemblance, and usually does not resemble the substrate.