In addition to inhibiting the first direct step of a pathway, does a feedback
inhibitor always inhibits the last step as well ?
The fact that a product is produced at the active site of the enzyme means that
it must be bound there momentarily before being released. Such binding does not
necessarily imply inhibition, as it may be much weaker than substrate binding.
Addition of high amounts of product will reverse a reaction by thermodynamic
(energy) considerations, but this does not constitute enzyme inhibition, just
reversal of the reaction direction.