Km: if this is a constant, does this mean that k1, k2, and
k3 are fixed in relation to one another?
k1, k2, and k3 are rate CONSTANTS. They are fixed values for any
particular enzyme and substrates and products. They are defined by how
the rate of a part of the reaction depends on the concentration of
reactants, such that:
k1[X] describes the influence of the concentration of X on the rate of
disappearance of X in a reaction of X < --> Y. Since X can also be
formed from Y as well as turn into Y, the total description of how X
changes with time is given by dX/dt = k1[X] - k2[Y], where the lower
case k's are the rate constants. The k's are not rates, they are
multiplication factors: the rate constants must be multiplied by the
concentration(s) of reactants present to get the individual rates for
each part of the reaction.