In Delta G calculations, are the concentrations of water and hydrogen ions also set equal to 1 as for the Delta Go calculations? A non-trivial question. For delta G, one should divide the concentration of products and reactants by the standard concentrations to obtain the "activities" of the reactants and products. It is these activities that are really meant to be used in the calculation of delta G. The starndard concentrations are 1 M for all but water and H+. For water the standard concentration is 55 M, and it is assumed that water is always at 55 M, so the H2O activity always comes out to 1, and is never a factor in the calculation. For H+ ions the concentration should be included and divided by 10^-7 M, which is the standard concentration of H+ (pH7) in biochemistry. Such a pH convention means you are calculating delta G'. This discussion goes beyond the level of this introductory course.