C2005/F2401 '08 -- Outline For Lecture 17-- Restriction Enzymes & Genetic Engineering

Last Posted/Updated 11/07/08 11:12 AM

I. Viral Life Cycle & Viral Genetics

A. Lysogeny

B. Retroviruses

C. Viral Crosses

II. Restriction Enzymes (Restriction Endonucleases)

A. Discovery -- how they protect bacteria from viruses

B. Examples

C. Significance -- use for cloning, forensics, etc.
 

III. Probes -- Why you need them and how you get them.

IV. Blots -- Uses and Procedure.

V. Molecular Cloning (if time)

A. What use is a clone of cells with a chimeric (recombinant) plasmid?

B. How to make a chimera (recombinant plasmid)

Next time:  Finish V. Then: How do you find the right clone? And what do you find out from cloning eukaryotic genes? (Introns)