C2005/F2401 '08 -- Outline for Lecture #18

Posted/last updated 11/07/08 11:13 AM

I. Making a library -- how you actually do cloning
 

II. Finding the 'right' clone in a library -- Where is the one with the insert you want?
 

III. What do you find if you clone a eukaryotic gene?

    A. Discovery  of Introns (Intervening DNA sequences)   

    B. Overview of Splicing

    C. Implications of splicing
 

IV. (If time) Intro to eukaryotic cell division: How does one eukaryotic cell make two?

    A. Problems caused by structure of eukaryotic genetic material -- more than one chromosome, chromosomes in nucleus, etc.

    B. Basic Solution -- 2 different states of the nucleus and the DNA

        1. Chromatin -- interphase state (between divisions)

        2. Chromosomes -- state during division (mitosis)

    C. The Cell Cycle -- M, G1, S & G2

    D. The Mitotic Chromosome Cycle -- Chromosomes, Chromatids & Centromeres

Next time: Finish IV above, if needed. Then the details of Mitosis (& then Meiosis) -- what happens at each major stage? What are the products?