C2006/F2402 '06 Outline Of Lecture #2 -- Updated
01/18/06 11:33 AM
© 2006 Deborah Mowshowitz, Department of Biological Sciences, Columbia University, New York NY
Want to see some pictures with the scanning EM? In Becker see fig. 16-5, 16A-1, 16-26, 17-1 etc. For some more pictures of biological specimens see http://www.le.ac.uk/bs/em/sem.htm; for a completely different use of the scanning EM see http://www.tfhrc.gov/pubrds/05may/03.htm.
Problems to do have been added in magenta italic bold. Problems listed below are posted on Courseworks (go to class files, and then to shared files).
-- Immunofluorescence & Freeze Etch ;
1B. You'll also need to refer to 1B -- Cytoskeleton
I. Immunofluorescence & the Cytoskeleton
A. How Components of Cytoskeleton are Visualized -- Immunofluorescence. See Becker Table 15-2 (22-2).
1. The "immuno" part: How antibodies are used as reagents to identify proteins (& other substances)
a. What are Antibodies & Antigens? Antibodies are made by vertebrates in response to foreign materials (antigens). Antibodies are always proteins; antigens can be proteins (as in all cases discussed here) or other substances.
b. Specificity. Each antibody (against a protein) binds to one protein or a very small number of similar proteins. (See Becker fig. 15-10 (22-12) for an example.)
c. Antibody Structure.
(1). Each antibody has a variable part -- complementary in fit to part of target (in this case, protein.)
(2). Each antibody has a constant part -- constant in all antibodies of that class from that species.
d. Use of Antibodies for detection. Many methods identify (or characterize) proteins by their function; antibodies identify proteins by their structure (irrespective of function). Therefore antibodies are often useful for detection of proteins that have no enzymatic activity (such as components of cytoskeleton).
e. Detection of Ab-Ag binding. How can you tell if an antibody has bound to its antigen? Clumping and complex formation is one way.
2. Role of Fluorescence See Becker Appendix, A-8 to A-11 (Guide to Microscopy p. 8-11); for pictures see Becker fig. 15-1 (22-1) or Purves 4.4 (4.3), 4.21.
a. Fluorescent materials emit light of one wave length when irradiated at a different wave length.
b. Fluorescent material can be located easily by irradiating sample (at one wave length) and seeing what part of sample "lights up" (emits light at a different wave length).
c. Small fluorescent groups or small fluorescent proteins (such as green fluorescent protein or GFP) can act as "tags" or probes -- can be attached to larger molecules such as antibodies, often without altering function of macromolecule.
3. Methods: How Fluorescent Antibodies are used (See handout 2A)
a. General Principle: Add fluorescent antibody, wash off unattached antibodies (not bound to antigen), irradiate, and look for light emission = site of fluorescent antibody (= site of target protein).
b. Direct Immunofluorescence -- Antibody with tag (fluorescent) sticks to target.
c. Indirect Immunofluorescence.-- Antibody #1 (without tag) sticks directly to target; secondary labeled (tagged) antibody sticks to constant part of first antibody. Advantages of indirect:
(1). Gives an amplification effect -- more tag or label per molecule of target protein.
(2). Requires only one labeled antibody to identify many proteins. Same labeled secondary antibody can be used to bind to ("light up") many different proteins.
(a). A different primary antibody is used for each target protein. (Not labeled -- no tag.) Variable part of primary antibody binds to specific part of target protein.
(b). The secondary antibody binds to the constant part of the primary antibody. Therefore the same (labeled or tagged) secondary antibody can bind to many different (unlabeled) primary antibodies.
B. How Cytoskeleton Discovered
1. How get (labeled) antibodies to MT and MF? Large, permanent structures made of tubulin and actin well known in specialized cells. Tubulin (from cilia & flagella) & actin (from thin filaments of muscle) purified; antibodies made to them. Fluorescent probes were attached to the antibodies, and antibodies were injected into living cells.
2. Cytoskeleton of MF & MT Identified -- Structures made of actin & tubulin found in in cytoplasm of almost all eukaryotic cells. (Not just in specialized cells & structures such as muscle, cilia, etc.)
a. Antibodies to tubulin bind to ("light up") MT.
b. Antibodies to actin bind to MF.
3. Implies two possible states of tubulin and actin:
a. Temporary (dynamic) -- no fixed state; monomers and polymers are in dynamic equilibrium as in cytoskeleton, spindle fibers, cleavage furrow.
b. Permanent -- form stable specialized structures such as cilia, flagella and muscle fibers. See Purves 4.23 (4.24) or Becker fig. 16-7 & 8 (23-7 & 23-8) for nice pictures of cilia.
4. IF found later in multicellular organisms. (Not present in unicellular eukaryotes.)
Look at problem 1-1.
II. Microfilaments & Microtubules
A. Overview of Structure -- See last lecture. For a summary of properties, see handout 1B or Becker Table 15-1 (22-1). For pictures, see Becker fig 15-1 (22-1). For diagrams, see handout 2B (& Becker 4-24). In Purves, see 4.21. Many nice pictures and additional details (which go beyond the scope of this course, but might be of interest) are in Becker Chap. 15 (22).
B. How each monomer forms a polymer (See handout 2B)
→ dimer → chain of dimers (protofilament) → rings of chains forming a tubule (usually 13 chains/tubule).
a. Monomer is globular. Globular monomer (G actin) forms chain of beads. Two chains twist around each other ---> polymer.
b. Polymer has polarity. Polymer looks symmetric (in standard picture) but isn't -- has "+" and "-" ends (like a chain of pop beads does). Nice pictures of how MF provide structural support to microvilli are in Purves 4.22 (4.23) and Becker figs. 15-20 & 21 (22-18 & 22-19).
c. Growth: Polymer grows preferentially at + end.
d. How drugs affect polymerization.
a. Two types of globular monomers -- alpha and beta.
b. How tubule forms: Alpha + beta
3. IF. Monomers are extended chains, not globular. Monomers are assembled into more extended chains and formed into a cable as explained below.
Look at problems 1-7 & 1-8 (A & B).
C. Role in movement -- See Purves 4.24 (4.25) or Becker fig. 16-3 (23-3).
1. IF not involved in movement (don't lengthen or shorten); Do assemble/disassemble during cell cycle.
2. MF, MT involved in movement. Primarily using a "motor molecule" (see handout 1B) that slides down fiber using a rachet mechanism (& splitting of ATP).
a. Two major motor molecules for tubulin/MT:
(1) Dynein -- Dynein carries material "in" toward the nucleus/cell body -- Dynein draws things in (toward the - end of MT).
(2) Kinesin -- Kinesin carries material "out" toward the edges of the cell -- to the cell "Korners" (toward the + end of MT).
b. One major motor molecule for actin/MF = myosin
3. Two major types of movement using motor molecules
a. Can have 2 fibers sliding past each other (motor is part of one fiber or in between the two)-- overall effect is to shorten/lengthen structure. See Purves 4-14 (a). Examples:
(1). Anaphase -- MT slide "out" to give longer spindle fibers and elongated spindle.
Note: Overall movement of chromosomes seems to depend both on sliding of some fibers past each other, and the change in length of other individual fibers. For details, see Becker.
(2). Telophase -- MF slide "in" forming cleavage furrow that divides cell in half.
b. Can have vesicle or large structure moving down a fiber (motor attached to vesicle) -- fiber acts as "railroad tracks" to direct vesicle toward one end of fiber. Example -- How vesicles move down axons of neurons. See Becker fig. 16-1, 16-2 & 16-5 (13-1, 23-2 & 23-5) or Purves 4.24 (b) & (c).
Neurons are nerve cells with long extensions (axons) that make connections (synapses) with other cells at the end of the axon. Materials are carried up and down the axon along MT between nucleus/cell body and synapse at end of axon. Direction of movement depends on whether motor molecule is dynein or kinesin.
Look at problems 1-9 to 1-11. See handout 1-B for effects of drugs mentioned.
III. IF's -- see handout 2B
A. How monomers form a polymer
; both monomers point in the same direction.
1. Monomers are extended, not globular -- see handout.
2. Two monomers → dimer
3. 2 dimers → tetramer; tetramer = fibrous basic subunit. Two dimers in a tetramer point in opposite directions.
4. Tetramers stick to each other by overlapping ends → protofilament.
5. Multiple protofilaments form a cable, probably 8 protofilaments across. Exact final structure unclear. One possibility: protofilaments form flat cable that twists into final structure. (fig. 15-25(22-25) of Becker = handout 2B.)
Note: Becker text says tetramer = protofilament; this appears to be an error. All other texts and Becker diagram describe protofilament as a long structure formed by many tetramers.
B. Not all IF's are the same. All IF's have similarities, and form similar structures, as above, but there are multiple IF genes and therefore multiple IF's with significant differences. (See Becker fig. 15-24 (22-24) = handout 2B)
1. Nuclear IF's -- Lamins. Lamins are a type of IF found in the nucleus. Do not confuse them with laminins (proteins found in the extracellular matrix). Lamins are the same in all cell types.
2. Cytoplasmic IF's
a. All IF's but lamins are cytoplasmic. (All MF & MT are cytoplasmic.)
b. Cytoplasmic IF's (not lamins) are tissue specific -- origins of cancers can be traced from the type of IF's they contain. That's because different tissues transcribe ("express") different IF genes.
C. IF's represent a protein/gene family. See Purves Ch 26 esp. pp. 516-519 (Ch. 24 esp. pp. 442-446) for more on evolution of gene families.
1. Proteins tend to occur in "families" -- groups of similar proteins.
2. Examples: all IF's (& their genes) are similar. All globins (Hb alpha chains, Hb beta chains, myoglobin) are similar to each other but very different from all IF's. All antibody chains are similar to each other but not to globins and IF's, and so on.
3. How do families form? All members of a family have a common evolutionary origin -- ancestral gene duplicated and copies diverged → family of related proteins.
a. Why do copies stay so similar? Sections of the protein (& corresponding sections of the gene) that were essential to IF formation were preserved in all duplicates -- mutations that caused loss or serious alteration of these sections were lost (= conservative selection against mutations that ruin function = selection against organisms that function worse than average).
b. Why do copies diverge? Alterations that allowed useful variations in function were preserved (= innovative selection for mutations that improve function = selection for organisms that function better than average).
4. How to 'fine tune' protein structure & get a group of similar, but different, proteins? By gene duplication to give a gene family OR alternative splicing of a single gene.
IV. Membrane Structure -- Any of this that is not finished will be covered in lecture #3.
A. Lipid part
1. Amphipathic nature of lipids (See Purves 5.2) -- there are multiple different "two headed" lipids -- each type has a different structure, but each has a hydrophobic end and hydrophilic end.
2. Amphipathic Lipids form a bilayer.
B. Protein part -- where are the proteins (relative to the lipid)? Is it a "unit membrane" or a "fluid mosaic?"
For "unit membrane" See Becker fig. 7-4 ; for fluid mosaic model see Becker fig. 7-5 (or 7-3) or Purves 5.1.
1. Use of freeze fracture/freeze etch procedure
a. E vs P faces of bilayer = surfaces you see if you crack bilayer open = inside of bilayer
(1). E face = inside of the monolayer that is closer to extracellular space
(2). P face = inside of the monolayer that is closer to protoplasm
b. What do you see on inside? (see handout 2A, bottom & Becker fig. 7-16 & 7-17 or Purves 5.3).
(1) Inside is not smooth -- shows proteins go through bilayer (implies "mosaic" model not unit membrane)
(2). More bumps (proteins) on P face than E face -- shows more proteins anchored on cytoplasmic (protoplasmic) side.
c. Sometimes see P face & E face in one picture. (See 2A). In this case, you are looking at P face of one membrane (from one cell) and E face of a different membrane from a neighboring cell. You are not looking at both sides of the same membrane.
d. Freeze fracture vs Freeze etch
(1). Freeze fracture = crack frozen sample open, examine in EM;
(2). Freeze etch = crack open, let some water sublime off, then look in EM. For some sample pictures, see Becker figs. 15-16, 15-21, 15-26, & 16-1. (22-19, 22-21, 22-26, & 23-1.)
C. Fluid mosaic model -- current idea of how proteins and lipids are arranged. See Becker fig. 7-5 (or 7-3) or Purves 5.1.
Next Time: Details of membrane structure; then on to transport -- how are small molecules moved across membranes?