C2006/F2402 '08 OUTLINE OF LECTURE #7
(c) 2008Dr. Deborah Mowshowitz, Columbia University, New York, NY. Last update 02/11/2008 06:16 PM
Handouts: 6A: Protein Transport (from last time); 7A = How proteins insert into ER membrane, & 7B -- Signal Hypothesis -- Co-translational Import 7C = Golgi & Lysosomes; not on web. (also has article from NY Times)
For a nice video (& explanation) of a pulse chase experiment, try this link: http://www.sumanasinc.com/webcontent/anisamples/majorsbiology/pulsechase/pulsechase.html
I. Sorting of Proteins to their Proper Place: Overview (See top of handout 6A & Becker fig. 22-14 (20-14) or Sadava 12.15 (12.14) -- terminology in Sadava is slightly different.)
A. What determines the fate (final location) of each individual protein? The amino acid sequence of the protein itself. The ability of each protein to reach its proper destination is built into the protein. The presence (or absence) of localization signals in the amino acid sequence of the protein is the determining factor.
1. What's a localization signal: a group of amino acids acting as an "address" or "tag" directing the protein to a particular destination.
2. Terminology: The localization signal or "tag" is often called a localization sequence (LS) or patch.
a. LS -- if it consists of a continuous section in the peptide chain.
b. Patch -- if it consists of a contiguous section in the folded protein. (But AA are not next to each other in the unfolded chain.)
3. Use of tags: The localization sequence/patch directs the protein to the ER, nucleus, etc. Several different localization sequences, which are read sequentially, may be needed to direct a protein to its proper destination. If no "tag" or special sequence/patch is present at all, the protein remains in the (soluble) cytoplasm.
B. The Big Divide -- Attach to the ER or not?
1. Ribosomes start to make protein first. Translation starts first; then ribosome location (attachment to ER or not) is determined by the sequence of the protein being made.
2. Which ribosomes go to ER: If protein has the right "tag" (a localization signal called a signal peptide (SP) or signal sequence) the ribosomes attach to the ER.
3. Which ribosomes stay in cytoplasm: If there is no SP, ribosomes remain "free" in the cytoplasm -- they do not attach to ER or any other membrane.
(Note: Becker says there are ribosomes translating mRNA in the nucleus. Maybe, maybe not. This finding is controversial -- the existence of nuclear ribosomes is not firmly established. So we are ignoring them.)
C. Fate of proteins made on free ribosomes
1. Soluble Cytoplasm -- the default location for a soluble protein. If there are no "tags" at all, proteins stay in the cytoplasm.
2. Organelles. If proteins have the right "tags" they can be imported post-translationally (after synthesis) into organelles (nuclei, mito, chloro or peroxisomes) that are NOT part of the endomembrane system.
3. Terminology: "free" means not attached to a membrane. All ribosomes making protein, "free" or not, are attached to mRNA.
D. Fate of proteins made on attached ribosomes -- these become part of the endomembrane system and/or leave the cell.
1. They enter the ER by co-translational import (and some post-translational import, esp. in unicellular eukaryotes). Protein can
- Remain inserted in membrane -- become a transmembrane single or multipass protein (details below)
- Pass all the way across the membrane and end up in lumen of ER (as soluble protein or peripheral, membrane associated protein on lumen side)
2. Most proteins travel from ER to Golgi (a few may remain in ER) -- vesicles carrying protein bud off ER, travel to Golgi and fuse with cis side of Golgi (side nearest to nucleus).
(1). Suppose domain X of a multipass integral membrane protein is on the inside of the ER, sticking into the lumen of the ER. When a vesicle forms off the ER, where will domain X be? Inside vesicle in lumen? On outside of vesicle in cytoplasm?
(2). When vesicle fuses with Golgi, where will domain X be? In lumen of Golgi? Sticking out into cytoplasm?
3. Most proteins are sorted and processed in the Golgi and packed into vesicles that bud off the trans side of Golgi (also called TGN = trans Golgi network).
4. Where do the proteins and/or vesicles go next?
(vesicles involved in regulated secretion) → area near plasma membrane
a. Secretory vesicles
(1). Vesicles fuse with plasma membrane only in response to signal (such as hormone, change in ion concentrations, etc.)
(2). Fusion results in: Release of contents outside cell and/or addition of material to cell membrane. Click here for animation #1 -- annotated & animation #2 -- larger but not annotated.
b. Default vesicles (vesicles involved in constitutive secretion) → plasma membrane → fuse automatically (constitutively) and release contents. Same as in (a) -- leads to addition of material to membrane or outside it. HOWEVER no signal is required for fusion. This is probably the "default" for proteins that are directed to the ER but have no additional directional information.
c.Vesicles containing hydrolases → Lysosomes (details to be discussed in future lectures).
d.Vesicles containing other enzymes → other parts of EMS (Some enzymes may stay in trans Golgi, but others bud off and go back to other parts of Golgi, ER, etc.)
Try problems 3-1, A & B, 3-13, & 3-16, A & B. You should be able to do 3-14, but you may have to look up the localization of some of the enzymes/proteins listed.
II. ER -- How does Co-translational Import Work?
A. Signal hypothesis -- How ribosomes get to the ER & Protein enters ER -- See handout 7B. Steps listed below refer to handout. See Becker fig. 22-16 (20-16) or Sadava fig. 12.16 (12.15).
1. What is the Signal Hypothesis? Ribosome unattached to ER starts making protein. (Step 1.) If nascent (growing, incomplete) peptide has a "signal peptide," then ribosome plus growing chain will attach to ER membrane, and growing chain will enter ER as it grows.
2. How does ribosome get to the ER?
a. Signal peptide (SP) = section of growing peptide (usually on amino end) does not bind directly to the ER. Binds to 'middleman' called SRP. (Step 2.)
b. SRP = signal recognition particle = example of an RNP (ribonucleoprotein particle)
- Role: temporarily blocks translation; ferries nascent protein to ER.
- Structure: SRP contains proteins + RNA = Large particle containing both ribonucleic acid & protein like a ribosome or spliceosome.
- Helps attach ribosome to ER, so growing chain can enter pore (translocon) in ER.
3. How does Growing Chain enter ER? Takes two steps (3 & 4)
a. ER has SRP receptor. Receptor is also called docking protein. SRP binds to SRP receptor/docking protein, not directly to pore. (Step 3.)
b. ER has Translocon (gated pore or channel through membrane) which allows growing chain to pass through membrane as chain is made. Pore is closed until ribosome with growing chain gets into position.
Note on terminology: The term "translocon" is used in (at least) 2 different ways. Sometimes it is used to mean only the channel itself, and sometimes it is used to mean the whole complex of proteins required for translocation of proteins across the membrane -- the channel, SRP receptor, etc. It should be clear from context which usage is meant at any one time.
c. Ribosome/translocon complex formation occurs. (step 4 = complex step involving several events)
- SRP is released, recycles -- GTP split
- Ribosome binds to pore/translocon
- Translocon opens & peptide enters (as loop).
- Ribosome resumes translation.
4. How does a new protein end up in the lumen?
. Translation (and movement through translocon) continues (step 5)
b. Translation (and translocation) are completed, translocon closes.(step 6)
c. Signal Peptidase cuts off signal peptide at arrow.(step 7)
Now try problem 3-4, especially part D. (If some of the parts are not obvious, wait until later.)
B. How do proteins cross or enter the ER membrane? (See handout 7A and/or fig. 22-17 (20-17) of Becker)
1. How proteins enter/pass through the membrane -- important points
a. SP probably forms loop not arrow. Loop enters channel (translocon) in membrane. SP loop is probably what opens (gates) the channel on the cytoplasmic side.
b. Protein enters as it is made. In humans, growing protein chains usually enter the ER as the chains are synthesized (co-translational import).
Note: In unicellular organisms, proteins often enter the ER after they are finished (post-translational import). Post translational import into the ER will be ignored here, but is covered at length in cell biology.
c. How do transmembrane proteins get anchored in the membrane? A hydrophobic sequence may trigger opening of the pore sideways, so protein slides out of pore, laterally, into lipid bilayer. These hydrophobic sequences are called 'stop-transfer' sequences and/or 'anchor' sequences.
d. Where will protein end up? Protein can go all the way through the membrane and end up as a soluble protein in the lumen (as in example above, on 7B) or protein can go part way through and end up as a transmembrane protein. Depends on sequence of protein.
2. Types of Proteins that can result (see handout 7A)
a. Soluble protein in lumen. Happens if protein passes all the way through the membrane and SP (on amino end) is removed, as above.
b. Integral membrane protein anchored in membrane by SP with no cytoplasmic domain. This happens if SP is on the amino end and is not removed.
c. Single Pass transmembrane protein -- get one of 2 possibilities:
(1). Type 1: Amino end is on lumen side of membrane (on E side); Carboxyl end is in cytoplasm (on P side of membrane)
One way this could happen: If SP is on amino end, and SP removed, and there is a hydrophobic sequence (acting as a stop-transfer sequence) in the middle of the peptide.
(2). Type 2: Carboxyl end is on lumen side of membrane (on E side); Amino end is in cytoplasm (on P side)
One way this could happen: If SP is in the middle, not on amino end. SP in this case is not removed -- it becomes the transmembrane domain of the protein. (SP doubles as stop-transfer sequence.)
d. Multipass transmembrane protein. (Requires one SP and several hydrophobic (start/stop) sequences.
(1). Hydrophobic sequences can stop the process (of moving through pore) and anchor protein in membrane, as explained above.
(2). Hydrophobic sequences in the middle of the peptide can restart looping → multipass protein. These are usually called "start-transfer" sequences.
(3). 'Start-transfer' and 'stop-transfer' sequences are probably equivalent. Role depends on where in protein they occur. (Both start- and stop-transfer sequences are also called 'topogenic sequences' as they determine the topology of the finished peptide.)
(4). Every SP is also a "start transfer sequence" but not every start/stop sequence is necessarily an SP. To act as an SP, a sequence must bind to the SRP. If a hydrophobic sequence does not bind the SRP, it will not direct ribosomes to the ER.
e. Lipid Anchored Proteins: Proteins to be anchored to lipids on the outside of the plasma membrane are generally made as follows: Protein is made on RER and inserted into the ER membrane. After the protein reaches the plasma membrane, the extracellular domain is detached from the rest of the protein and attached to lipid. (Proteins to be anchored to the plasma membrane on the inside are made on cytoplasmic ribosomes.) See Becker if you are curious about the details.
Now finish problem 3-1 & try 3-2, A -C. To test yourself on structure/insertion of integral membrane proteins, try problem 3-3.
III. What Else Happens in/on the ER?
A. What happens inside ER
1. Terminal SP usually removed by signal peptidase (enzyme) inside ER.
2. Folding of protein -- requires chaperones.
a. Chaperones (also called chaperonins) -- proteins needed to assist in protein folding. Chaperones are used every time a protein remains unfolded or becomes unfolded to cross a membrane (or refolds on the other side). Different chaperones are found in different parts of the cell.
b. Chaperones are of two major types (families) -- HSP 60 (forms barrel) or HSP 70 (binds to hydrophobic regions). Differ in molecular weight (60 K vs 70 K) and mode of action. (See an advanced text if you are curious about the mechanisms.)
c. The major chaperone inside the ER is a member of the HSP 70 family, also called "BiP"
d. Why are chaperones named HSP 60, HSP 70? HSP = heat shock protein. Chaperones, aka HSP's, are made in large amounts after exposure to high temperatures. (That's how they were first discovered.)
3. Enzymatic Modifications. The appropriate enzymes inside the ER catalyze the following:. In eukaryotes, all S-S bonds are formed in proteins inside the ER. Proteins made in the cytoplasm do not have S-S bonds. Cytoplasmic proteins do contain cysteines and have free SH groups.
a. Making of S-S bonds
b. Start of N-glycosylation (oligosaccacharides are added to the N of the amide of asparagine side chains -- called N glycosylation.) See Becker fig. 12-7 if you are curious about the biochemical details.
c. Removal of SP as above.
4. Some proteins stay in ER (in lumen or membrane); most move on to Golgi.
5. What happens to proteins in ER that do not fold properly? See Becker p. 755-757 (733-734).
a. Transport to cytosol -- Unfolded proteins are transported back to the cytosol (through the translocon -- mechanism unknown).
b. Ubiquitin addition -- in cytosol, proteins to be degraded are marked for destruction by addition of a multiple molecules of a small protein called ubiquitin to side chains of lysine. (See Becker or advanced texts if you are interested in the enzymatic details.)
c. Role of Proteasome = a large protein complex in cytosol that degrades ubiquitinylated proteins to fragments, at expense of ATP. Major site of degradation of intracellular proteins. (Proteins from outside are generally degraded in lysosomes.)
d. What goes to the proteasome? Proteins that are misfolded, damaged, or have served their function.
A major proportion of all proteins made in cell do not fold properly and are degraded.
Destruction of many proteins is regulated -- level of protein activity can be controlled by protein degradation as well as by rate of synthesis, feed back of activity, modification, etc. More details and/or examples to follow.
e. 2004 Nobel Prize for Chemistry was awarded to Aaron Ciechanover, Avram Hershko and Irwin Rose for the discovery of the ubiquitin/proteasome system.
B. What happens on outside of ER (besides protein synthesis)
1. Lipid synthesis --(exchange) proteins.
a. Insertion: Lipids made and inserted on cytoplasmic side (cytoplasmic leaflet) of membrane by enzymes attached to/in membrane.
b. Flipping: Enzymes ('flippases') required to move amphipathic lipids from one leaflet (P side) of membrane to other leaflet (E side).
c. Transport: Lipids can reach parts of cell not connected to ER through vesicles and/or transport
2. Some detoxifications and other reactions are catalyzed by proteins on the cyto side of ER. See text for details if interested.
To review the structure and function of the ER, try problem 3-4.
1. Two sides of stack
a. cis/forming face (side closest to nucleus & ER)
b. trans/maturing face (away from nucleus)
2. Three basic parts or compartments in a stack
a. CGN (cis-Golgi network) or cis Golgi -- may include fusing vesicles
b. medial cisternae (sacs) -- part in between 'cis' and 'trans' Golgi
c. TGN (trans-Golgi network) or trans Golgi -- may include budding vesicles
3. Different marker enzymes/functions found in different parts. (See Becker figs 12-5 & 12-6) Enzymes unique to any one cell organelle or compartment are called 'marker enzymes' = their presence is a 'marker' for the presence of that compartment or organelle.
4. Sacs in stack connected by vesicle traffic -- not completely clear which way transport vesicles go or what they carry. (See below.) It is clear that newly made protein and lipid passes through the Golgi from the cis face to the trans face, as shown on this animation.
C. Function -- what reactions take place inside Golgi?
-- oligosaccharide that was added to glycoproteins in ER is modified. Oligosacch. is attached to "N" of amide side chains of asparagines (asn's).
1. Finish N glycosylation
2. Do O glycosylation of glycoproteins. Sugars are added to "O" of the hydroxyl of the side chain of ser & thr.
3. Assemble sugars of proteoglycans (linear chains of repeating sequence = GAGs)
4. Concentrate, sort proteins. This occurs at trans face (TGN). Different areas of Golgi have receptors that trap proteins going to different destinations.
To review how proteins are directed to the right place and modified in the ER and Golgi, try problem 3-2.
V. Transport Through the Golgi -- Are cisternae stationary or do they progress?
1. What is known: Transport vesicles can bud off one sac (cisterna) of the Golgi and fuse with another in the same stack. In vitro, vesicles can fuse with cisternae of another stack. (See problem 3-10.)
2. Three big Issues
a. Direction of vesicle traffic: Which way do the vesicles go?
1. cis to trans = forward = anterograde?
2. trans to cis = backward = retrograde?
3. Both? Older models assumed it was forward, but current evidence indicates it is both -- some vesicles go in one direction and some the other, as shown in Becker fig. 12-8.
b. What do the vesicles carry?
1. Cargo proteins -- Newly made proteins from the ER, and/or
2. Modification Enzymes -- Used in the Golgi to modify the newly made cargo proteins
c. Do cisternae move? What carries the newly made material from cis to trans, the cisternae or the vesicles?
B. Models -- see handout 7C.
1. "Vesicle Transport Model" or Stationary Cisternae Model
a. Transport vesicles move primarily forward (anterograde) -- towards trans face. For an animation of this process, see here.
b. Vesicles carry cargo -- vesicles carry newly made proteins from one part of Golgi to next part for additional modifications.
c. Sacs of Golgi (& their characteristic enzymes) stay put -- newly made proteins (cargo) pass from sac to sac by means of vesicles.
d. Enzyme composition of each sac stays the same. Each part stays in the same place and holds on to its characteristic ('marker') enzymes. It is the substrates of the enzymes (the newly made cargo proteins) that pass through, carried by the vesicles from sac to sac.
2. "Cisternal Maturation Model" = a modified Progression Model
a. Transport vesicles move primarily retrograde -- towards cis face.
b. Vesicles carry enzymes to modify and sort proteins. They do not carry the newly made proteins from the ER.
c. Sacs of Golgi move, carrying newly made proteins & lipids inside. New sacs are constantly formed at the cis face from material transported from the ER. Old sacs are lost from the trans face as they age.
d. Enzyme composition of each sac changes with time. The enzyme composition of each individual sac is constantly changing as it ages and passes from cis to trans face. However the characteristic enzymes found in the sacs at each position of the Golgi (cis, medial & trans) remain the same, because the enzymes are "passed back." The vesicles retrieve the enzymes of "older" sacs (closer to the trans face) and carry them back to newer sacs (closer to the cis face).
3. Connection Model (FYI) -- another possibility is that the Golgi sacs are actually connected (although they seem to be separate), and that proteins move back and forth from one sac to the next, although net bulk flow of newly made proteins is from cis to trans. There isn't much evidence or enthusiasm for this model, but some recent data using genetically modified protein tagged with GFP has been interpreted in support of this model.
4. What really happens? The models above are not mutually exclusive, so a hybrid model is possible. Only new experiments generating new data will settle the question of how material actually moves. Not all materials may move through the Golgi in the same way. For a review of the models and the data as of 1998 (the 100th anniversary of the discovery of the Golgi) see Coming to Grips with the Golgi.
To review traffic through the Golgi, try problem 3-10.
VI. Lysosomes: an example of sorting at the end of the Golgi -- If we don't get to this we'll do it next week.
A. How proteins get to lysosomes (See Becker fig. 12-9) -- Normal Pathway (steps refer to numbers on top of handout 7C)
1. How do hydrolases reach the Golgi?2. How are hydrolases identified & tagged for transport to lysosomes?
a. Enzyme synthesis: Hydrolases are made on ribosomes bound to ER. Enzymes enter ER co-translationally (as they are made).
b. Transport to Golgi: Hydrolases are transported in vesicles to Golgi. Steps 1 & 2.
a. Localization Signal -- Most hydrolases destined for lysosomes have a special sequence/patch.
b. Reading the LS -- Enzyme(s) recognize the sequence/patch and add Mannose-6-P (N-glycosylation starts in ER by addition of standard oligosaccharide. Modification of standard sugars to M6P occurs in Golgi -- this modification only happens to proteins with the proper amino acid sequence = soluble hydrolases bound for lysosomes) Step 3.
3. Role of M6P Receptor -- how is the tag recognized?4. Sorting after the Golgi
a. Binding the M6P -- Receptor in special part of trans Golgi binds proteins with M6P. Step 4.
b. Sorting in trans Golgi -- Proteins with M6P and their receptors accumulate in coated pits (step 5) and bud off (step 6) and go to a sorting vesicle/endosome (step 7).
a. Sorting vesicle/Endosome sorts multiple types of receptors and hydrolases. Same as what happens during RME when receptors and ligands are sorted. (step 7)
b. Recycling: M6P Receptors recycle back to Golgi (steps 8A & 10); vesicles with hydrolases add to old lysosome or form new one (steps 8B & 9). Note that 8A & 8B are equivalent to the same numbers on the handout of RME. 8B goes to the lysosome and 8A recycles back to the membrane from which it came.
c. SNAREs. How do various vesicles fuse when they reach the proper target? There are matching transmembrane proteins (SNAREs) on the target membrane and the vesicle membrane. The cytoplasmic domains of the proteins are complementary, and pair up with each other. See SNARE hypothesis in Becker, p. 348-349 (352-353), if you are interested in more details.
B. Lysosomal diseases -- next time -- what if hydrolases are not made and/or do not reach the lysosome? How do enzymes get to peroxisomes, nuclei and mitochondria?