Topics discussed in class & suggested references

(in addition to class notes and only to supplement them)

Linkage

2-Factor Mapping: Griffiths Chapter 4 pp. 116-125; Hartwell Chapter 5 pp. 113-117 & 121-125.

Obtaining recombinants with minimum effort (from trans/ from cis/ from A individuals): Class notes.

3-Factor Mapping and interference (textbook method): Griffiths Chapter 4 pp. 125-127; Hartwell Chapter 5 pp. 126-129.

3-Factor Mapping (alternative more efficient method): Handout.

Translocations and Linkage

Definitions, Inversions, Pseudolinkage, Balancers: Griffiths Chapter 15 pp. 496-502; Hartwell Chapter 13 pp. 452-458.

Other strategies

SNPs, RFLPs and tandem repeated sequences: Griffiths Chapter 4 pp. 127-129 and Chapter 12 pp. 398-402; Hartwell Chapter 11 pp. 378-380 & 383-387.

Use of transposons for mapping genes in C. elegans

Chromosome identification: Class notes.

Position within the chromosome: Class notes.

LOD score and uses

Definition & use: Griffiths Chapter 4 pp. 133-134; Hartwell Chapter 11 pp. 392-393.

Use of Hybrid Cells for mapping

Definition: Class notes.

Use: Class notes.

Exchange of genetic material in bacteria (Hartwell references are recommended)

Conjugation, Fertility Factor & Recombination: Griffiths Chapter 5 pp. 152–161; Hartwell Chapter 14 pp. 496-503 & 505-506.

Mapping: Griffiths Chapter 5 pp. 161-165; Hartwell Chapter 14 pp. 503-505.

Benzer and the unit of recombination: Griffiths Chapter 5 pp. 166-170; Hartwell Chapter 7 pp. 208-213.

Using deletions for rapid mapping: Hartwell Chapter 7 pp. 208-213.

Generalized Transduction: Griffiths Chapter 5 pp. 170-172; Hartwell Chapter 14 pp. 506-508.

Specialized Transduction: Griffiths Chapter 5 pp. 172-174; Hartwell Chapter 14 pp. 508-509.

F' factors: see above under Fertility Factor.

Ordered Tetrads (Hartwell references are recommended)

Mapping & centromere mapping: Griffiths Chapter 4 pp. 129-131; Hartwell Chapter 5 pp. 132-140.

Recombination

Octads & gene conversion: Griffiths Chapter 14 pp. 473-474; (Hartwell Chapter 5 pp. 138-140.) Hartwell Chapter 6 pp. 179-180.

Models: Griffiths Chapter 14 pp. 474-476; Hartwell Chapter 6 pp. 180-185; Handout.

co-conversion & polarity: Class notes.

Mosaics

Definition and generation: Hartwell Chapter 5 pp. 140-142 & Reference D pp. 820-822.

Factors affecting the result: Class notes.

Techniques for examining gene expression

RNA in situ hybridization: Hartwell Chapter 19 p. 661.

LacZ as a reporter gene: Griffiths Chapter 16 pp. 528-529; Hartwell Chapter 18 pp. 565-566.

GFP as a reporter gene: Griffiths Chapter 16 pp. 537-538; Hartwell Reference C p. 798.

Antibodies and protein fusions: Griffiths Chapter 11 p. 353 & Chapter 16 pp. 528-529; Hartwell Chapter 19 pp. 661-662.

Reverse Genetics (Griffiths references are recommended)

Definition: Griffiths Chapter 16 pp. 530-535; Hartwell Reference C p. 800.

Mutating Genes (Hartwell references are recommended)

Site directed recombination in bacteria: Griffiths Chapter 16 p. 531; Hartwell Reference p. 511.

Mice: Griffiths Chapter 11 pp. 372-375; Hartwell Reference E p. 852.

Site directed recombination in flies: Griffiths Chapter 11 p. 371; Hartwell Reference D pp. 825-826.

Sib selection: Class notes.

RNAi: Griffiths Chapter 16 pp. 533-534; Hartwell Reference C pp. 798-799 & Reference D p. 826.

Transposition (Griffiths references are strongly recommended)

Transposition in Eukaryotes: Griffiths Chapter 13 pp. 424-429 & pp. 434-438; Hartwell Chapter 13 pp. 460-465.

Transposition in Prokaryotes: Griffiths Chapter 13 pp. 429-433; Hartwell Chapter 14 pp. 493-494 & 495-496.

Transposition as a tool: Griffiths Chapter 13 pp. 438-440 & Chapter 16 p. 530; Hartwell Reference D pp. 819-820.