G4008
– G-protein Coupled Receptors
Fall
2003
1.
GPCR
Structure
Rhodopsin crystal, electron density maps
Readings
The papers included here are 3 research papers and a review article.
Of the three research papers please read the Palczweski
et al. paper (Crystal Structure...) carefully. The two papers by Schertler et al. are very technical in nature and you
should not get bogged down in methodological details. Go over them, but mostly
concern yourselves with the results of the experimental measurements and their
comparison with the Crystal Structure paper.
2.
Structure
Function Analysis
Readings
Mutational analysis
Binding
Spin label
SCAM
Chimeras
Correlated mutational analysis
3.
Expression
a.
Dimers
b.
Co-factors
c.
Post-translational
modifications
4.
Modulation
a.
Trafficking,
phosphorylation, densensitization,
RGS proteins
Jennifer J. Figs 1,2
Yun Z., Figs 3,4
BaRK Structure
Kristin R.. Figs 1,2
Carmelle N. Figs 3,4
Ashley L. Figs 5,6,7
P
siters on BaRK
Ben J. Figs 1,2
Newlon S. Fig 3
Bruna B, fig 4,5
Christina D. Fig 6,7
Neil G. Fig 8,9
G-prot bind to BaRK
Robin M. Figs 1,2
Ellen P. figs 2,3
P site removal
Masoud T. Figs 1,2
Alexander A. Figs 3,4
Arrestin PNAS 88
Denise W. fig1-3
Arrestin & GPCRs
Sung Min P. Figs 1,2
Elizabeth S. Figs 3,4
Andriy K. Figs 5,6
Andrea L. figs 7,8
5.
Genomics
a.
SNPs,
families, evolution
6.
Discovery
a.
HTS,
orphans, FRET, etc.
Meeting Dates: September 2, 4, 8, 11, 15, 23, 29.
October 1, 7, 13, 20 (22 or 23)
Paper due: Dec 4-10