Chromosomes: karyotype, euploid, aneuploid, heteroploid, pseudodiploid, haploid
chromosome, chromatid, sister chromatids, homologues, centromere, telomere.
metacentric, submetacentric, acrocentric, telocentric, metaphase chromosomes.
G-bands, C-bands, Q-bands, secondary constriction, FISH, cytocentrifuge
p, q = short, long arms (e.g., chromosome band 3p2; 6pter [ter = terminus])
stemline chromosome number (1s, 2s)
Cell culture and growth control: Primary, secondary culture; senescence, crisis, passage number.
Establishment; established, permanent, immortal cell line; telomerase
Confluence, quiescence, transformation, neoplastic transformation.
Density dependent growth, anchorage independent growth, suspension culture, growth in agarose
Saturation density, topoinhibition, plating efficiency = cloning efficiency.
Exponential growth, log phase, stationary phase, G₁, S, G₂ M, G₀.
Popular cell lines: L-cells, 3T3, cos, CHO, V79, BHK, Vero, HeLa, 293, HT1080, cos, AGMK, human diploid fibroblasts
Some specialized lines: Hep2, HII, Friend cells, MELC, PC12, S49, L1210, 10T?, F9, ES, MDCK, Raji, NT40
Some popular viruses: SV40, polyoma, RSV, BPV, CMV, HSV, HIV, polio, vaccinia, influenza (flu)
Cell fusion: cell hybrids: recessive, dominant, co-dominant
segregation: concordant, discordant
syntenic, synteny
heterokaryon, homokaryon; cytoplast, karyoplast
Mutation: transition, transversion, frameshift, inversion, translocation, epigenetic,
hemizygous; functional hemizygosity; partial haploidy; homozygosis
HAT, BUdR, BrdU, TK, APRT, HPRT, HGPRT, DHFR, TG, AG, AzaA, IMP, LDH, GAPD, AzaC, MTX
Expression period, LD₅₀, mutation frequency vs. mutation rate, fluctuation test, metabolic cooperation
Transfection: Transfection, transformation, lipofection, electroporation, CaPhosphate transfection
Transient transfection, stable transfection, permanent transfection, pooled permanents.
Copy number, position effect, MAR, SAR, insulators, boundary elements, LCR
DNA: replicon, amplicon, heterochromatin, euchromatin, Alus, SINEs, LINEs, pseudogenes, processed pseudogenes
DNA methylation, hemimethylation, CpG islands, HpaII and MspI, DpnI
Lab words:Lab enzymes: T4 polymerase, Klenow, PolI, RNase , RNase T1, RNase A, T4 ligase, Taq polymerase, S1, mung bean nuclease, Bal31, T4 kinase, terminal transferase, CIP, AP, beta-gal, luciferase, CAT, dhfr, hprt, GFP
Molecular biology: PCR, RT-PCR, forward primer, nested primer, tailed primer, RACE, differential display
primer extension, Southern, Northern, Western, Far-Western, RNase protection, pulsed gel electrophoresis
epitope (antigenic determinant), flu-tagged, FLAG, IP, co-IP, GST-fusion, His-tagged
Gels:native gel, denaturing gel, urea-gel, alkaline gel, formaldehyde gel, agarose, polyacrylamide, low-melt agarose
Instruments:spectrophotometer, scintillation counter, Phosphorimager, densitometer, luminometer, fluorimeter, FPLC, HPLC
Microscopy:biotin, avidin, digoxigenin, excitation and emission filters, CCD, confocal microscope, pseudocolor, propidium iodide, fluorescein, Texas Red, rhodamine, DAPI
Biochemistry:Km, Ka, Kd, Kcat, off-rate, Cot, Tm, deltaGo