Columbia University

Department of Biological Sciences

W3004/W4004: Cellular and Molecular Neurobiology

 

Fall 2006

Lectures:  Tu/Th 4:10-5:25; 417 International Affairs Building

Recitations: 

Graduate students:  Tuesday 5:40 in room 1000 Fairchild                      TA: Josh Dudman

Group I:                       Tuesday 5:40 in room 700 Fairchild                       TA: Angela Gee       

Group II:                      Thursday 5:40 in room 900 Fairchild                      TA: Kristen Werner

Group III:                     Tuesday 3:00 in room 900 Fairchild                       TA: Gulsen Surmeli

Group IV:                    Monday 6:30 in room 700 Fairchild                        TA: Dara Sosulski

 

 

Instructor:

Jian Yang, jy160@columbia.edu                

Office Hours by appointment through e-mail;

 

Teaching Assistants (the dream team!):

Josh Dudman            jtd2001@columbia.edu
Angela Gee               alg2021@columbia.edu

Dara Sosulski            dls2128@columbia.edu

Gulsen Surmeli          gs2241@columbia.edu

Kristen Werner          kmw2115@columbia.edu

 

 

Required Text:        Principles of Neural Science, 4th ed., 2000.

                                    Eric Kandel, James Schwartz and Thomas Jessell

                        McGraw-Hill Companies, New York

 

Supplemental Texts:

Neuroscience, 3rd ed., 2004, Purves et al.

Sinauer Associates, Sunderland, MA

 

From Neuron to Brain, 4th ed., 2001, Nicholls et al.

Sinauer Associates, Sunderland, MA

 

 

Recitations:  A strong emphasis is placed on readings from the primary literature, which will be discussed in weekly recitations.  Students will read and discuss in depth selected research papers.  Attendance of the recitations is mandatory and active participation will count in the final grade.  Materials discussed in the recitations will be included in the exams. 

 

Class Attendance:  Attendance is not taken, however material will be presented in lectures that is not available in the text and which will appear on examinations

 

Exams and Grading: Exams and Grading:  Three exams and a final examination are given.  Each exam will cover material since the previous exam.  The final examination is entirely on papers from the primary literature.  The lowest grade of the three periodic exams will be dropped (students may choose to take only two tests).  The remaining exams will each contribute 30% towards the semester grade; the final will contribute 35% and is required.  5% will come from recitation attendance and participation. No make-up exams will be allowed except in cases of serious illness or personal crisis and the student is required to present a letter from the undergraduate dean as well as other supporting evidence (such as doctor’s notes, airline tickets etc.).

                                                        

 

SCHEDULE

 

Part I: Membrane Electrical Properties

 

Sept. 5:           Lecture 1.  Introduction, Cell Biology of the Neuron                    

                        (Chapter 2, 4)

Sept. 7:           Lecture 2.  Ionic Basis of the Resting Membrane Potential       

(Chapter 7)

 

No recitation first week of class

 

Sept. 12:        Lecture 3.  Ion Channels                                                                

 (Chapter 6)

Sept. 14:        Lecture 4.  Ion Channels                                                                

(Chapter 6)

 

Recitation 1:  Doyle et al., “The structure of the potassium channel: molecular basis of

K+ conduction and selectivity”.  Science 280, 69-77 (1998).

 

Sept. 19:        Lecture 5.  Targeting, Trafficking and Localization of Ion Channels

 slides

Sept. 21:        Lecture 6.  Passive Electrical Properties of the Neuron

(Chapter 8)

 

Recitation 2:  Problem set #1

 

Sept. 26:        Lecture 7.  Ionic Basis of the Action Potential                             

(Chapter 9)

Sept. 28:        Lecture 8.  Generation and Propagation of Action Potentials   

(Chapter 9)

 

Recitation 3: Problem set #2

 

Oct. 3:             Off

 

Oct. 5:             EXAM I (Lectures 1-8, Recitations 1-3)

 

No recitation this week

 


Part II: Synaptic Transmission

 

Oct. 10.           Lecture 9.   Synaptic Transmission: Overview, Neuromuscular Junction

(Chapter 10, 11)                                                                                           

Oct. 12.           Lecture 10.  Synaptic Transmission: Neuromuscular Junction, CNS

(Chapter 11, 12)                                                                               

 

No recitation this week

 

Oct. 17.           Lecture 11.  Synaptic Transmission: CNS                                     

(Chapter 12)

Oct. 19.           Lecture 12.  Synaptic Transmission: Slow Synapses                

(Chapter 13)

 

Recitation 4: Biederer et al., “SynCAM, a synaptic adhesion molecule that drives synapse assembly”.  Science 297, 1525-1531 (2002).

 

Oct. 24.         Lecture 13.  Presynaptic Mechanisms: Transmitter Release     

(Chapter 14)

Oct. 26.         Lecture 14.  Synaptic Plasticity: Long-term Potentiation 

(Chapter 63) 

 

Recitation 5:  Fernandez-Chacon et al.  “Synaptotagmin I functions as a calcium regulator of release probability”.  Nature 410, 41-49 (2001).

 

This paper is required for only graduate students, but undergraduate students may also find it interesting to read. 

 

Oct. 31.        Lecture 15.  Biochemistry of Neurotransmitters                            

                    (Chapter 15)     

Nov. 2.        Lecture 16.  Synapse Formation and Regeneration

                        (Chapter 55)            

  

Recitation 6: Shi et al., “Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation”. Science 284, 1811-1816 (1999).

 


Part III: Sensory Physiology

 

Nov. 7.            Off (election day)

Nov. 9.            EXAM II (Lectures 9-15, recitations 4-6) 

 

No recitation this week

 

Nov. 14.          Lecture 17.  Introduction: Coding of Sensory Signals

                         (Chapter 21)                                                                                    

Nov. 16.          Lecture 18.  Phototransduction and Color Vision

(Chapter 26)                                                                                     

 

Recitation 7: Palczewski, K. et al. Crystal structure of rhodopsin: A G protein-coupled receptor.” Science 2000 Aug 4;289 (5480):739-45.

 

Nov. 21.          Lecture 19.  Pain                                                                            

                        (Chapter 24)

Nov. 23.          Off (Thanksgiving)

 

Recitation 8: Zheng J. et al. “Prestin is the motor protein of cochlear outer hair cells.”
Nature 2000 May 11;405
(6783):149-55.

 

Nov. 28.          Lecture 20.  Mechanotransduction in the Ear                              

                        (Chapter 30, 31)

Nov. 30.          Lecture 21.  Olfaction                                                                                                                      (Chapter 32) and reading   

 

Recitation 9: Zhang, X. and S. Firestein (2002). "The olfactory receptor gene superfamily of the mouse." Nat Neurosci 5 (2): 124-33.

 

 Dec. 5.           Lecture 22.  Taste                          

                        (Chapter 32) and reading   

 

 Dec. 7.           EXAM III (Lectures 17-22, recitations 8-10)

 

 

Dec. ???.     Final exam

 

The final exam will consist of three sets of questions on selected research articles. It is suggested that you read and analyze these papers prior to the exam, since the exam will be closed-book; i.e. you CANNOT look at the articles, notes or textbook during the exam. You may discuss these papers with other students, but not with the instructors or the TAs.