W3040 - Laboratory in Cell and Developmental Biology

Dr. Uri Shanas
700 Fairchild
uas2@columbia.edu

Office Hours: Monday 3-4pm, Friday 1 1-l2noon, 744 Mudd

*Interested students should submit an application (forms in Dept. Office, 600 Fairchild) before the first day of classes. Leave completed application in the folder in room 600 or in Dr. Shanas’s mailbox, located on the 7th floor of Fairchild.

Introduction:
Class meets for a lecture each Tuesday evening, 5:30-7:OOpm, in room 700 Fairchild. The group will be separated into 2 sections, one of which will start their lab work immediately after the lecture, the other will begin on Thursday afternoon at 1:30pm. Students should allow 3.5 hours to complete their work in the evening & afternoon sessions, and it will also be necessary to come in to the lab occasionally on other days of the week. Students will spend an average of 7 hours per week in the l ab and 5 hours studying and completing reports. The lab is located in 753 Fairchild extension, actually in the Mudd building.

Course Activities:
We will use the mouse as a Mammalian model to study functional histology and neuronal activity and Xenopus laevis (an amphibian) to study morphogenesis.

Students will develop their own experiments and will process organs and tissues for histology. In addition students will learn basic histology features of selected organs (such as gut, gonads, brain).

Techniques:
Dissection of a mouse.
Fixing, embedding, cutting and staining tissues.
Use of the a microscope.
Photography through the microscope
Immunocytochemistry.

Prerequisites:
Students should have already taken Intro to Mol. & Cell Bio. with Prof. Moshowitz and Gibber.

Reading:
To be announced

Reports and Exams:
Three reports, 50 points each.

Final exam, 50 points.

Schedule:

Sep 8: Inroduction to the lab, safety, Introduction to histology: The cell; basic tissue types, fixation, design and start.

 

Sep 15. Dissection and fixation of mouse tissues Opening the mouse
Identifying and fixing the following tissues: heart, lungs, liver, pancrease, spleen, duodenum, stomach, duodenum
, jejuneum, caecum, colon, urinary bladder, kidney, adren, spin al cord, brain, harderian gland, gonads.

 Microscopy: theory of resolution Basic concept in optics and microscopy, Setting the microscope, use of lOOx oil immersion objective. measuring microscopic objects

Sep 22.Embedding the tissues in paraffin. Film strips of histology
Histology of commercially prepared slides (identifying tissues, cells, measuring, drawings).

Sep 29: Yom Kippur

Oct 6: Use of a microtome
Cutting and mounting

Oct 13: Staining the slides with different stains.
Covering. Continue histology

Oct 20: Histology of the slides prepared (several different organs and the experimental organ).
Photography
Writing a final report for the first experiment.
Planing experiment 2

 

Oct 27: Experiment 2.
perfusion, embedding

Nov 3: Election Day

Nov 10: Introduction to immunocytochemistry
Cuffing
Immunocytochemistry

Nov 17: Immunocytochemistry
Counter staining
Report on experiment 2.

Nov 24: Xenopus Development

Dec 1: Xenopus Development
Final assignment

Dec8: Final assignment

Dec 15: Recitation Session

Dec 22: Final Exam