W3040- Laboratory in Cell and Developmental Biology
Dr. Uri Shanas
Office Hours: Monday 3-4pm, Friday 1 1-l2noon, 744 Mudd
*Interested students should submit an application (forms in Dept. Office, 600 Fairchild) before the first day of classes. Leave completed application in the folder in room 600 or in Dr. Shanas’s mailbox, located on the 7th floor of Fairchild.
Students will develop their own experiments and will process organs and tissues for histology. In addition students will learn basic histology features of selected organs (such as gut, gonads, brain).
Reports and Exams:
Final exam, 50 points.
Sep 8:Inroduction to the lab, safety, Introduction to histology: The cell; basic tissue types, fixation, design and start.
Sep 15. Dissection and fixation of mouse tissues Opening the mouse
Identifying and fixing the following tissues: heart, lungs, liver, pancrease, spleen, duodenum, stomach, duodenum , jejuneum, caecum, colon, urinary bladder, kidney, adren, spin al cord, brain, harderian gland, gonads.
Microscopy: theory of resolution Basic concept in optics and microscopy, Setting the microscope, use of lOOx oil immersion objective. measuring microscopic objects
Sep 22.Embedding the tissues in paraffin. Film strips of histology
Histology of commercially prepared slides (identifying tissues, cells, measuring, drawings).
Sep 29: Yom Kippur
Oct 6: Use of a microtome
Cutting and mounting
Oct 13: Staining the slides with different stains.
Covering. Continue histology
Oct 20: Histology of the slides prepared (several different organs and the experimental organ).
Writing a final report for the first experiment.
Planing experiment 2
Oct 27: Experiment 2.
Nov 3: Election Day
Nov 10: Introduction to immunocytochemistry
Nov 17: Immunocytochemistry
Report on experiment 2.
Nov 24: Xenopus Development
Dec 1: Xenopus Development
Dec8: Final assignment
Dec 15: Recitation Session
Dec 22: Final Exam