Home Page for ChloŽ Bulinski's Lab

ChloŽ Bulinski

Columbia University
Biological Sciences
804A Fairchild Center, M.C. 2450
New York, N.Y. 10027

Return to Biology HomePage

The polymerization and depolymerization dynamics of microtubules (MTs) are subject to exquisitely precise regulation in vivo, probably owing to the fact that small perturbations in MT dynamics are sufficient to arrest cell division, differentiation and motility processes. In fact, numerous chemotherapeutic drugs target MT dynamics in order to halt tumor cell proliferation, metastasis or viability; the slight alteration of MT dynamics produced by these drugs is often sufficient to launch cells on an apoptotic cascade. Our laboratory studies MT-associated proteins (MAPs) that play various roles in the regulation of MT dynamics during the cell cycle and differentiation, as well as in
response to MT-antagonistic drugs. These proteins are also capable of modulating MT functions, such as mitosis and vesicle transport. One MAP we study, MAP4, is the most abundant MAP present in organisms ranging from the worm, Caenorhabditis elegans, to humans.  MAP4 has been hypothesized to be an important regulator of MT dynamics. We and others had previously shown that increased expression of MAP4, including novel isoforms, contributes to increased MT stability in muscle cells. Interestingly, we found that MAP4 has a dual function in proliferating cells:  it stabilizes MT dynamics and regulates MT polymer level (1). We recently used site-directed mutations in MAP4 phosphorylation sites to test the hypothesis that MAP4's phosphorylation state regulates its 
activity at the G2 : M transition of the cell cycle. Analysis of 
phosphorylation-defective mutants demonstrated that in vivo phosphorylation of MAP4 by cdc2 kinase reduces MAP4's capacity to stabilize MTs, and these phosphorylation events are necessary for efficient progression from interphase into mitosis, when the interphase MT array must be disassembled and the dynamics of the MTs increased (2). Our laboratory had previously shown that another MAP, ensconsin, which is present in a variety of human cells, does not regulate microtubule dynamics. Recent experiments revealed that increased expression of ensconsin is deleterious to cells treated with MT-stabilizing chemotherapeutics, e.g., Taxol (3), while a variety of biochemical experiments 
suggested that ensconsin's binding to MTs might be altered by Taxol treatment. To investigate this hypothesis, we developed 
a method called fluorescent speckle microscopy (FSM) that allows one to image single molecules in vivo; it optimizes visibility of fluorescent molecules in living cells and reveals assembly dynamics, movement, and turnover of protein assemblies (4). We used FSM to follow the dynamics of ensconsin molecules' association/dissociation with MTs in vivo. We found that ensconsin normally existed in dynamic association with MTs, but in cells that were Taxol-treated, or had their MT dynamics halted by other means (i.e., depleting cellular ATP levels or extracting cells with detergent) ensconsin became more statically associated with the MT(5). Quantifying ensconsin's residence time along the length of a MT by another technique, fluorescence recovery after photobleaching (FRAP) we found that the t1/2 of ensconsin's MT binding is rapidly increased up to 50-fold by decreasing the dynamics of the MTs to which the ensconsin is bound (5). These results suggest that ensconsin may function as a 'sensor' of MT dynamics, signaling to the cell that deleterious MT stabilization has occurred. We are currently testing this hypothesis.
Another means by which cells can monitor MT dynamics is by monitoring the post-translational modification state of the tubulin within their MTs. For example, the protomers within stable MTs become modified by detyrosination. During myogenesis, MTs become extensively detyrosinated, and we have recently shown that, even when the MT stabilization takes place normally, this MT detyrosination is necessary for myogenic morphogenesis and expression of muscle-specific genes (6).




Representative Publications

(1) Nguyen, H.L., Gruber, D. and Bulinski, J.C. 1999. Microtubule-associated protein 4 (MAP4) regulates assembly, protomer-polymer partitioning and synthesis of tubulin in cultured cells. J. Cell Sci. 112:1813-1824.
Article (.pdf)

(2) Chang, W., Gruber, D., Chari, S., Kitazawa, H., Hamazumi, Y., Hisanaga, S.I., and Bulinski, J.C. 2001. Phosphorylation of MAP4 affects microtubule properties and cell cycle progression. J. Cell Sci. 114:2879-2887.  Article (.pdf)

(3) Gruber, D., Chang, W., Faire, K., and Bulinski, J.C. 2001. Abundant expression of the microtubule-associated protein, ensconsin (E-MAP-115), alters the cellular response to Taxol. Cell Motil. Cytoskel. 49: 115-129.  Article (.pdf)

(4) Waterman-Storer, Desai, A., Bulinski, J.C. and Salmon E.D. 1998. Fluorescent speckle imaging: Visualizing the movement, assembly, and turnover of macromolecular assemblies in living cells. Current Biology 8:227-230.  Article (.pdf)

(5) Bulinski, J.C., Odde, D., Howell, B., Salmon, E. D., and Waterman-Storer, C. M. 2001. Rapid dynamics of the binding of ensconsin to microtubules in vivo. J. Cell Sci. 114 (21), Article (.pdf)

(6) Chang, W., Webster, D.R., Salam, A.A., Gruber, D, Prasad A., Eiserich, J.P., and Bulinski, J.C. 2002. Alteration of the C-terminal Amino Acid of Tubulin Specifically Inhibits Myogenic Differentiation. J. Biological Chemistry.  227 (34). Article (.pdf)

Back to the top | Return to Biology