Chalfie's Abstract

Science 263: 802-5 (1994)[94135254]

Green fluorescent protein as a marker for gene expression.

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward & D. C. Prasher

Department of Biological Sciences, Columbia University, New York, NY 10027.

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

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