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Transfer 1 ml, 1 ml, 1 liter of liquid from 1 vessel
to another? |
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Make 500 ml of 1 M TrisHCl, pH 7.8 from
scratch without a recipe? |
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Make 500 ml of 100 mM TrisHCl, pH 7.8, 50 mM NaCl,
100 mM MgCl2 from stock solutions with a recipe?
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Make the above solution without a recipe?
|
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Digest 2 mg of pBR322 with EcoRI and run the result
on a 0.8% agarose gel without a protocol? |
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Design and execute a subcloning experiment –
starting from two samples of plasmid DNA and ending with one engineered DNA construct? |
|
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Follow new protocols for molecular biology
experiments that you have never done before? |
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Decide which protocol to use and what
reagents to acquire from where for a new project? |
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Carry one line of tissue culture cells for
one month? |
