Use a 1 10 scale to answer the following questions:

1: with ease

4: can do with some careful thinking

7: probably can do.  Not sure.

10:  I have no idea how

If you were left alone in a well-equipped and stocked lab, can you

Transfer 1 ml, 1 ml, 1 liter of liquid from 1 vessel to another?

Make 500 ml of 1 M TrisHCl, pH 7.8 from scratch without a recipe?

Make 500 ml of 100 mM TrisHCl, pH 7.8, 50 mM NaCl, 100 mM MgCl2 from stock solutions with a recipe?

Make the above solution without a recipe?

Digest 2 mg of pBR322 with EcoRI and run the result on a 0.8% agarose gel without a protocol?

Design and execute a subcloning experiment starting from two samples of plasmid DNA and ending with one engineered DNA construct?

Follow new protocols for molecular biology experiments that you have never done before?

Decide which protocol to use and what reagents to acquire from where for a new project?

Carry one line of tissue culture cells for one month?