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Comparative Proteomics Center
Background
The proteome is the expressed protein complement of a cell, matrix, organelle, tissue, organ, or organism. It includes all isoforms and posttranslational variants and varies with time. The overall technical approach in proteomics was enabled by two major technical advances: the ability to fully sequence genomes and the ability to analyze proteins by mass spectrometry. Comparative proteomics defines the differences in expression of proteins among different biological states (e.g., control vs. treatment, healthy vs. disease, specific genotype vs. wild type) or for affinity purifications. The Department of Biological Sciences of Columbia University has established the Comparative Proteomics Center to apply these emerging technologies to a wide range of biomedical research studies. We use label-free shotgun profiling as well as gel-based techniques to perform these comparisons.
The proteome is the expressed protein complement of a cell, matrix, organelle, tissue, organ, or organism. It includes all isoforms and posttranslational variants and varies with time. The overall technical approach in proteomics was enabled by two major technical advances: the ability to fully sequence genomes and the ability to analyze proteins by mass spectrometry. Comparative proteomics defines the differences in expression of proteins among different biological states (e.g., control vs. treatment, healthy vs. disease, specific genotype vs. wild type) or for affinity purifications. The Department of Biological Sciences of Columbia University has established the Comparative Proteomics Center to apply these emerging technologies to a wide range of biomedical research studies. We use label-free shotgun profiling as well as gel-based techniques to perform these comparisons.
The focus of the Comparative
Proteomics Center is the identification of proteins with differential
occurrence in cells, tissues or in affinity purifications...
...we have applied this technique to many different research problems.
We are always interested in doing
collaborative projects with research groups located across all campuses
of Columbia University and at other institutions across the nation.
Click on the working with us link for more information.
