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Comparative
Proteomics Center
Equipment
Applied Biosystems Voyager De Pro MALDI-TOF
This matrix-assisted laser desorption ionization time-of-flight mass spectrometer with delayed extraction is used to for identification of proteins from 2D gels using peptide mass fingerprinting (peptide mass mapping) in reflectron (high resolution mode). In linear mode another detector is used to determine the mass of intact proteins.
Applied Biosystems Voyager De Pro MALDI-TOF
This matrix-assisted laser desorption ionization time-of-flight mass spectrometer with delayed extraction is used to for identification of proteins from 2D gels using peptide mass fingerprinting (peptide mass mapping) in reflectron (high resolution mode). In linear mode another detector is used to determine the mass of intact proteins.
Mascot Proteomics
Database Server
Mascot Version 2.1 (Matrix Science Ltd.) running on an HP xw4400 with Intel Core2 Due Extreme x8600 processor, 4 Gb memory and 1 TB hard disk space. This database server allows comparison between mass spectra and protein databases to facilitate the identification of proteins.
Mascot Version 2.1 (Matrix Science Ltd.) running on an HP xw4400 with Intel Core2 Due Extreme x8600 processor, 4 Gb memory and 1 TB hard disk space. This database server allows comparison between mass spectra and protein databases to facilitate the identification of proteins.
Isoelectric
Focusing
Three GE Healthcare IPGphor isoelectric focusing units allows separation of proteins on the basis of charge. These units are used in the first dimension of 2D gel electrophoresis in support of the DIGE technique. Our three units are equipped with computer logging of run progress for quality assurance.
Three GE Healthcare IPGphor isoelectric focusing units allows separation of proteins on the basis of charge. These units are used in the first dimension of 2D gel electrophoresis in support of the DIGE technique. Our three units are equipped with computer logging of run progress for quality assurance.
SDS PAGE
Electrophoresis
The GE Healthcare Ettan DALTsix SDS PAGE electrophoresis unit with power supply and external refrigerated circulator allows separation of proteins on the basis of size. This unit is used in the second dimension of 2D gel electrophoresis in support of the DIGE technique. This unit is equipped with computer logging of run progress for quality assurance.
The GE Healthcare Ettan DALTsix SDS PAGE electrophoresis unit with power supply and external refrigerated circulator allows separation of proteins on the basis of size. This unit is used in the second dimension of 2D gel electrophoresis in support of the DIGE technique. This unit is equipped with computer logging of run progress for quality assurance.
Typhoon Trio
Laser Imager
The Typhoon Trio (GE Healthcare) is a laser scanner used for fluorescence imaging. This system includes 3 lasers and allows imaging of three discrete images with negligible spillover for the DIGE workflow. This unit is currently under lease and installed in the lab
The Typhoon Trio (GE Healthcare) is a laser scanner used for fluorescence imaging. This system includes 3 lasers and allows imaging of three discrete images with negligible spillover for the DIGE workflow. This unit is currently under lease and installed in the lab
DyCyder
2D
This software package (GE Healthcare) is used for the analysis of relative expression of proteins based on fluorescence signals. It uses the images produced by the Typhoon imager to detect relative differential regulation of proteins. This software is currently under lease and installed in the lab.
This software package (GE Healthcare) is used for the analysis of relative expression of proteins based on fluorescence signals. It uses the images produced by the Typhoon imager to detect relative differential regulation of proteins. This software is currently under lease and installed in the lab.
Ettan Spot
Picker
This robotic system (GE Healthcare) picks protein spots from 2D gels and transfers them to bar-coded 96-well plates using spot coordinates generated by the DeCyder software.
This robotic system (GE Healthcare) picks protein spots from 2D gels and transfers them to bar-coded 96-well plates using spot coordinates generated by the DeCyder software.
Bruker Esquire 3000+
ion trap with nanospray source and Agilent 1100 series LC with autosampler
Peptides are separated by HPLC and introduced in this mass spectrometer with electrospray ionization at nanospray flow rates. Fragmentation takes place in this 3D ion trap and proteins are identified by comparison to the NCBI database using the local Mascot database server.
Peptides are separated by HPLC and introduced in this mass spectrometer with electrospray ionization at nanospray flow rates. Fragmentation takes place in this 3D ion trap and proteins are identified by comparison to the NCBI database using the local Mascot database server.
