The concept is based on the development of projects supporting
the ongoing research programs of collaborating laboratories. This strategy
leverages the specialized expertise and equipment in proteomics available at the
center, and has the potential for significantly advancing the research projects
of the collaborators.
Comparative proteomics is a complex technology
regardless of the particular techniques used. Our strategy is to conduct
the proteomics work within the center as comprehensive collaborative
projects. The work done includes a complete package of activities designed
to complete a mutually agreed upon scope of work. Pilot (exploratory)
projects of minimal scope can also be performed. A typical project
includes:
Planning and comprehensive consultation
Training and consultation on sample preparation
Provision of tested and certified reagents and protocols for protein
sample preparation
Consultation on experimental design
Publication quality, fully analyzed, validated and interpreted data
Advice about limitations of results
Validation and summarization of results
Consultation on results interpretation
Consultation in higher level systems biology interpretations
Supported for the development of grant proposals including the development
of preliminary data
Participation in project meetings
Comprehensive support in preparing data for publication
Support for manuscript revision in response to reviewer comments
Support for revision of grant proposals in response to reviewer comments
Consultation on strategies for validation of results by corroborative
methods not involving proteomics methods
The center maintains expertise
and equipment for the many components of the workflow including sample
preparation, sample prefractionation, DIGE chemistry, isoelectric focusing, SDS
PAGE electrophoresis, fluorescence laser imaging, image analysis, robotic spot
excision, robotic sample processing, mass spectrometry, data analysis and
presentation. It is impractical for each collaborating laboratory to
acquire the equipment and skills needed for this specialized work.
A
Proteomics Advisory Committee aids the director in setting policies for the
center and in prioritizing activities.
Funding a New
Project
Funds for executing the projects are derived from project or
program grants awarded to the collaborating PI. These can be existing
projects or new projects proposed with support of the center. In the
latter case help with grant proposals is available from the center
director. The support and development of comprehensive shared efforts such
as these is a challenge to modern life science. Potential collaborators
should review a paper written by the Executive Board of the Association of Biomolecular Resource
Facilities that deals with these challenges.
Interested in beginning a new project?
The first step is
to contact the director to discuss your possible project. Also watch our
education and news pages for notices of seminars and talks that may provide some
ideas about how this technology may be useful in your
work.