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| FACULTY BIOGRAPHY |
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| Alexander Tzagoloff |
| Alan H Kempner Professor |
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| Web companion to the paper: "SIT4 Regulation of Respiration in S. cerevisiae"
Propagation of mitochondria depends on the expression of a small number of mitochondrial genes and a much larger pool of genetic information resident in nuclear DNA. Current estimates suggest that in the budding yeast Saccharomyces cerevisiae, close to 400 nuclear genes (referred to as PET genes) encode proteins with functions directly relevant to the maintenance of respiring mitochondria. While the functions of the mitochondrial gene products are well understood, the same cannot be said of the nuclear counterparts. An unexpectedly large proportion of PET genes have turned out to selectively affect the synthesis of individual respiratory complexes such as cytochrome oxidase and the proton translocating ATPase complex. In addition to coding for known subunit polypeptides of these heteroligomeric enzymes, PET genes govern processing of mitochondrial pre-mRNAs, translation of the mature mRNAs, and a host of post- translational events crucial for the production of the final active complexes. The functions of nuclear gene products involved in promoting late steps in assembly of the respiratory chain complexes, ensuing translation, import, and membrane insertion of all the constituents are the main focus of ongoing studies in our laboratory.
MedLine Listing of Dr. Tzagoloff's Publications |
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Representative Publications
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- Helfenbein KG, Ellis TP, Dieckmann CL, Tzagoloff A. (2003 ) ATP22, a Nuclear Gene Required for Expression of the F0 Sector of Mitochondrial ATPase in Saccharomyces cerevisiae. J Biol Chem. 278(22): 19751-6. Article
- Gin P, Hsu AY, Rothman SC, Jonassen T, Lee PT, Tzagoloff A, Clarke CF. (2003 Apr 29 [Epub ahead of print]. ) The saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin dependent monooxygenase required for coenzyme Q biosynthesis. J Biol Chem. Article
- Jin C, Barrientos A, Tzagoloff A. (2003 ) Yeast dihydroxybutanone phosphate synthase, an enzyme of the riboflavin biosynthetic pathway, has a second unrelated function in expression of mitochondrial respiration. J Biol Chem. 278(17): 14698-703. Article
- Barrientos A, Pierre D, Lee J, Tzagoloff A. (2003 ) Cytochrome oxidase assembly does not require catalytically active cytochrome C. J Biol Chem. 278(11): 8881-7. Article
- Carlson CG, Barrientos A, Tzagoloff A, Glerum DM. (2003 ) COX16 encodes a novel protein required for the assembly of cytochrome oxidase in Saccharomyces cerevisiae. J Biol Chem. 278(6): 3770-5. Article
- Nobrega MP, Bandeira SC, Beers J, Tzagoloff A. (2002 ) Characterization of COX19, a widely distributed gene required for expression of mitochondrial cytochrome oxidase. J Biol Chem. 277(43): 40206-11. Article
- Barros MH, Tzagoloff A. (2002 ) Regulation of the heme A biosynthetic pathway in Saccharomyces cerevisiae. FEBS Lett. 516(1-3): 119-23.
- Beers J, Glerum DM, Tzagoloff A. (2002 ) Purification and characterization of yeast Sco1p, a mitochondrial copper protein. J Biol Chem. 277(25): 22185-90. Article
- Barros MH, Nobrega FG, Tzagoloff A. (2002 ) Mitochondrial ferredoxin is required for heme A synthesis in Saccharomyces cerevisiae. J Biol Chem. 277(12): 9997-10002.
- Barrientos A, Korr D, Tzagoloff A. (2002 ) Shy1p is necessary for full expression of mitochondrial COX1 in the yeast model of Leigh's syndrome. EMBO J. 21(1-2): 43-52. Article
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