Small molecule dimerization inhibitors of HIV protease

Jean Chmielewski
Department of Chemistry
Purdue University



As more information on macromolecular structure unfolds, it has become obvious that interprotein interactions are a ubiquitous and fundamental aspect of biological activity. Whereas the nature of these protein-protein interactions is becoming better understood, rational approaches to inhibiting these interactions are still in their infancy.

Using dimeric HIV-1 protease as a template, we have developed a strategy to inhibit dimerization based on the crosslinking of interfacial peptides derived from the protease. In the current study, we endeavor to identify the essential residues of a crosslinked, interfacial peptide inhibitor of HIV-1 protease, and obtain a minimal structure that maintains the essential features of dimerization inhibition.