Laura Kaufman

Department of Chemistry, Columbia University
3000 Broadway, mail code 3128
New York, NY 10027

work:+1 212-854-9025

+1 212-932-1289

kaufman@chem.columbia.edu

Kaufman Research Group
Selected Publications

Bio in Brief
We endeavor to understand the structure, dynamics, and mechanical properties of systems that are variously (and loosely) described as crowded, frustrated, or jammed. In these systems, the particles of interest, whether they be as small as molecules (on the order of 10-10 m) or as large as cells (on the order of 10-5 m), are frustrated in their rotational and/or translational motion due to details of the structural, dynamical, and/or mechanical properties of their surroundings. Systems displaying frustrated dynamics include both molecular and colloidal supercooled liquids and glasses, as well as biological systems (the motion of macromolecules in biopolymer networks, as well as the motion of cells in tissue, demonstrates aspects of frustrated dynamics). In molecular and colloidal glassy systems, we wish to study fundamental issues concerning jammed dynamics, such as whether there are structural manifestations of the dramatic dynamical slowdown that occurs around a glass transition. In the biological systems we address, we ask more practical questions, such as how confinement in cells and tissues influences cell mobility and growth.

Because the length and time scales over which these frustrated dynamics take place differ substantially between molecular and colloidal and biological systems, we employ a wide range of spectroscopic and microscopic techniques together with theoretical modeling to probe and understand these systems. We use single-molecule-spectroscopy to elucidate the behavior of individual molecules in glassy molecular systems, while we use laser scanning microscopy together with particle tracking techniques to study similar behaviors in colloidal glassy systems. Nonlinear microscopies and one-photon fluorescent microscopy are used in concert with microrheological techniques to elucidate these dynamics in biological systems. The techniques we employ have one principal similarity: they are not ensemble measurements that average over different local environments in the systems. Instead, these techniques probe these different local environments, or spatial heterogeneities, in detail to elucidate if and how they cause (or result from!) the jammed or frustrated dynamics in these systems. To further study spatial and dynamical heterogeneities in such systems, we also develop new spatio-temporally resolved techniques that are analogous to time-resolved spectroscopic techniques, but are performed on much smaller focal volumes (mm3), and thus are ideal for colloidal and biological systems, which are heterogeneous on that length scale.

Publications

S.A. Mackowiak and L.J. Kaufman. "When the heterogeneous appears homogeneous: Discrepant measures of heterogeneity in single molecule observables," J. Phys. Chem. Lett. 2, 438-442 (2011).

S.A. Mackowiak, L.M. Leone, and L.J. Kaufman. "Probe dependence of spatially heterogeneous dynamics in supercooled glycerol as revealed by single molecule microscopy," Phys Chem Chem Phys. 13, 1786-1799 (2011).

Y. Yang, S. Motte, and L.J. Kaufman. "Pore size variable collagen gels and their interaction with glioma cells," Biomaterials. 31, 5678-5688 (2010).

S.S. Gallagher, C. Jing, D.S. Peterka, M. Konate, R. Wombacher, L.J. Kaufman, R. Yuste, and V.W. Cornish. "A trimethoprim-based chemical tag for live cell two-photon imaging," ChemBioChem. 11, 782-784(2010).

J.C. Conrad, H.M. Wyss, V. Trappe, S. Manley, K. Miyazaki, L.J. Kaufman, A.B. Schofield, D.R. Reichman, and D.A. Weitz. "Arrested fluid-fluid phase separation in depletion systems: Implications of the characteristic length on gel formation and rheology," J. Rheol. 54, 421-438 (2010).

Z. An, C.B. Gluck, M.L. Choy, and L.J. Kaufman. "Suberoylanilide hydroxamic acid limits migration and invasion of glioma cells in vitro," Cancer Lett. 292, 215-227 (2010).

S.A. Mackowiak*, T.K. Herman*, and L.J. Kaufman. "Spatial and temporal heterogeneity in supercooled glycerol: Evidence from wide field single molecule imaging," J. Chem. Phys. 131, 244513 (2009)

Y. Yang, L.M. Leone, and L.J. Kaufman. "Elastic moduli of collagen gels can be predicted from two dimensional confocal microscopy," Biophys. J. 97, 2051-2060 (2009).

J.Y. Lee, B.H. Hong, W.Y. Kim, S.K. Min, Y. Kim, M.V. Jouravlev, R. Bose, K.S. Kim, I.-C. Hwang, L.J. Kaufman, C.W. Wong, P. Kim, and K.S. Kim. "Near-field focusing and magnification through self-assembled nanoscale spherical lenses," Nature. 460, 498 - 501 (2009).

Z. An, K. Kavanoor, M.L. Choy, and L.J. Kaufman, "Polyelectrolyte Microcapsule Interactions with Cells in Two and Three Dimensional Culture," Coll. Surf. B: Biointerfaces (Accepted)

Y. Yang and L.J. Kaufman, "Rheology and Confocal Reflectance Microscopy as Probes of Structure and Mechanical Properties during Collagen and Collagen/Hyaluronan Self Assembly," Biophys. J. 96, 1566-1585 (2009)

T.K. Herman, S.A. Mackowiak, and L.J. Kaufman. "High power light emitting diode based set-up for photobleaching fluorescent impurities," Rev. Sci. Inst. 80, 016106 (2009)

B.M. Rubenstein and L.J. Kaufman, "The Role of Extracellular Matrix in Glioma Invasion: A Cellular Potts Model Approach," Biophys. J. 95, 5661-5680 (2008)

R. Chatterjee, N.C. Panoiu, K. Liu, Z. Dios, M.B. Yu, M.T. Doan, L.J. Kaufman, R.M. Osgood, and C.W. Wong, "Achieving Subdiffraction Imaging through Bound Surface States in Negative Refraction Photonic Crystals in the Near-Infrared Range," Phys. Rev. Lett. 100, 187401 (2008)

R. Zangi, S.A. Mackowiak, T. Herman, K. Kavanoor, and L.J. Kaufman "Towards Elucidating the Role of the Probe in Single Molecule Experiments in Supercooled Liquids," Reports of The Institute of Fluid Science 19, 17-22 (2007)

R. Zangi and L.J. Kaufman. "Frequency-dependent Stokes-Einstein Relation in Supercooled Liquids," Phys. Rev. E 75, 105501 (2007)

R. Zangi, S.A. Mackowiak, and L.J. Kaufman. "Probe Particles Alter Dynamic Heterogeneities in Simple Supercooled Systems," J. Chem. Phys. 126, 104501 (2007)

C. Guo and L.J. Kaufman. "Flow and Magnetic Field Induced Collagen Alignment," Biomaterials 28, 1105-1114 (2007)

L.J. Kaufman and D.A. Weitz. "Direct Imaging of Attractive and Repulsive Colloidal Glasses," J. Chem. Phys. 125, 074716 (2006)