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Wei Min

MC 3116

212-851-9433

wm2256@columbia.edu

Min Research Group
Selected Publications

Research Summary

Biophotonics is one of the fastest growing fields at the interface of physical science and life science. The newly emerging optical microscopy tools promise to make major contributions in unraveling biological processes that are localized in space, dynamic in time, and complex in nature. The Min group is dedicated to develop novel and powerful optical bio-imaging techniques and to apply them to visualize and understand dynamic behaviors of bio-molecules in live cells and organisms.

The current research is focused on the following two major directions:

(1) detect and study single-molecule dynamics in individual living cells by developing next-generation super-sensitive fluorescence microscopy; and

(2) label-free imaging of non-fluorescent small molecules, such as neurotransmitters and metabolites, by applying the recently developed nonlinear coherent Raman microscopy. When combined with molecular biology and genetics, this research would find exciting applications in neuroscience and metabolism biology.


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Publications

Y.-T. Kao, X. Zhu and W. Min. "Protein flexibility mediated coupling between photoswitching kinetics and the surrounding viscosity of a photochromic fluorescent protein", Proc. Natl. Acad. Sci. USA, 109, 3220 (2012).

L. Wei, Z. Chen and W. Min. "Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging", Biomed. Opt. Express,  (2012) in press

W. Min. "Label-free optical imaging of non-fluorescent molecules by stimulated radiation",  Curr. Opin. Chem. Biol., 15, 831, (2011)

X. Zhu and W. Min. "Frequency-domain phase fluorometry in the presence of dark states: a numerical study", Chem. Phys. Lett., 516, 40, (2011)

E. Gatzogiannis, X. Zhu, Y.-T. Kao, W. Min*. "Observation of frequency-domain fluorescence anomalous phase advance due to dark state hysteresis", J. Phys. Chem. Lett., 2, 461, (2011). * Corresponding author

M. C. Wang*, W. Min*, C. W. Freudiger, G. Ruvkun and X. S. Xie*. "RNAi screening for fat regulatory genes with SRS microscopy", Nature Methods, 8, 135, (2011). *Co-corresponding authors

W. Min, C. W. Freudiger, S. Lu and X. S. Xie. "Coherent nonlinear optical imaging: Beyond fluorescence microscopy", Annu. Rev. Phys. Chem., 62, 506, (2011)

C. W. Freudiger, W. Min, G. R. Holtom, B. Xu, M. Dantus and X. S. Xie. "Highly specific label-free molecular imaging with spectrally tailored excitation stimulated Raman scattering (STE-SRS) microscopy", Nature Photonics, 5, 103, (2011)

W. Min*, S. Lu*, S. Chong, R. Rahul, G. H. Holtom and X. S. Xie. “Imaging chromophores with undetectable fluorescence by simulated emission microscopy”, Nature, 461, 1105, (2009). *equal contribution

W. Min*, S. Lu*, M. Rueckel,. G. R. Holtom and X. S. Xie. “Near-degenerate four-wave-mixing microscopy”, Nano Lett., 9, 2423, (2009). *equal contribution

C. W. Freudiger*, W. Min*, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang and X. S. Xie. “Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy”, Science, 322, 1857, (2008). *equal contribution

W. Min, S. Lu, G. R. Holtom and X. S. Xie. “Triple-resonance coherent anti-Stokes Raman scattering microspectroscopy”, ChemPhysChem, 10, 344, (2009)

J. Lu*, W. Min*, J.-A. Conchello, X. S. Xie and J. W. Lichtman. “Super-resolution laser scanning microscopy through spatio-temporal modulation”, Nano Lett., 9, 3883, (2009). *equal contribution

W. Min, X. S. Xie and B. Bagchi. “Role of conformational dynamics in kinetics of an enzymatic cycle in a non-equilibrium steady state”, J. Chem. Phys., 131, 065104, (2009)


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