Virginia Cornish
Helena Rubinstein Professor
Department of Chemistry

work : +1 212-854-5209

Cornish Group



84. N. Ostrov, M. Jimenez, S. Billerbeck, J. Brisbois, J. Matragrano, A. Ager, V.W. Cornish. "A modular yeast biosensor for low-cost point-of-care pathogen detection." Sci Adv., 3, e1603221 (2017).

83. L. Wei, Z. Chen, L. Shi, R. Long, A.V. Anzalone, L. Zhang, F. Hu, R. Yuste, V.W. Cornish, W. Min. "Super-multiplex vibrational imaging." Nature, 544, 465-470 (2017).

82. R. Rogawski, I.V. Sergeyev, Y. Li, M.F. Ottaviani, V.W. Cornish, A.E. McDermott. "Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags." J. Phys. Chem. B, 121(6), 1169-1175 (2017).


81. A.V. Anzalone, Z. Chen, V.W. Cornish. "Synthesis of photoactivatable azido-acyl caged oxazine fluorophores for live-cell imaging." Chem. Commun., 52, 9442-9445 (2016).

80. X. Hu, C. Jing, X. Xu, N. Nakazawa, V.W. Cornish, F.M. Margadant, M.P. Sheetz. "Cooperative Vinculin Binding to Talin Mapped by Time-Resolved Super Resolution Microscopy." Nano Lett., 16(7), 4062-4068 (2016).

79. J.D. Boeke, G. Church, A. Hessel, N.J. Kelley, A. Arkin, Y. Cai, R. Carlson, A. Chakravarti, V.W. Cornish, L. Holt, F.J. Isaacs, T. Kuiken, M. Lajoie, T. Lessor, J. Lunshof, M.T. Maurano, L.A. Mitchell, J. Rine, S. Rosser, N.E. Sanjana, P.A. Silver, D. Valle, H. Wang, J.C. Way, L. Yang. "The Genome Project-Write." Science, 353(6295), 126-127 (2016).

78. A.V. Anzalone, A.J. Lin, S. Zairis, R. Rabadan, V.W. Cornish. "Reprogramming eukaryotic translation with ligand-responsive synthetic RNA switches." Nat Methods, 13, 458-458 (2016).


77. M.T. Englander, J.L. Avins, R.C. Fleisher, B. Liu, P.R. Effraim, J. Wang, K. Schulten, T.S. Leyh, R.L. Gonzalez Jr., V.W. Cornish. “The Ribosome Discriminates the Structure of the Amino Acid at its Peptidyl Transferase Center.” Proc. Natl. Acad. Sci. USA, 112, 6038-6039 (2015).


76. W.S. Yang, R. SriRamaratnam, M.E. Welsch, K. Shimada, R. Skouta, V.W. Viswanathan, J.H. Cheah, P.A. Clemons, A.F. Shamji, C.B. Clish, L.M. Brown, A.W. Girotti, V.W. Cornish, S.L. Schreiber, B.R. Stockwell. “Regulation of Ferroptotic Cancer Cell Death by GPX4.” Cell, 156, 317-331 (2014).

75. T.Y. Wang, L.J. Friedman, J. Gelles, W. Min, A.A. Hoskins, V.W. Cornish. “The Covalent Trimethoprim Chemical Tag Facilitates Single Molecule Imaging with Organic Fluorophores.” Biophys. J., 106, 272-278 (2014).


74. M.D. Harton, L.M. Wingler, V.W. Cornish. “Transcriptional Regulation Improves the Throughput of Three-Hybrid Counter Selections in Saccharomyces cerevisiae.” Biotechnol. J., 8, 1485-1491 (2013).

73. C. Jing, V.W. Cornish. “Design, Synthesis and Application of the Trimethoprim Based Chemical Tag for Live-Cell Imaging.” Curr. Protoc. Chem. Biol., 5, 131-155 (2013).

72. Z. Chen, V.W. Cornish, W. Min. “Chemical Tags: Inspiration for Advanced Imaging Techniques.” Curr. Opin. Chem. Biol., 17, 637-643 (2013).

71. C. Jing, V.W. Cornish. “A Fluorogenic TMP-tag for High Signal-to-Background Intracellular Live Cell Imaging.” ACS Chem. Biol., 8, 1704-1712 (2013).

70. N. Ostrov, L.M. Wingler, V.W. Cornish. “Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination.” Methods Mol. Biol., 978, 187-203 (2013).

69. A.R. Shepard, R.E. Conrow, M. Rezwan, K. Rutschmann, I.-H. Pang, D. Auerbach, R.P. SriRamaratnam, V.W. Cornish. “Identification of PDE6D as a Molecular Target of Anecortave Acetate Via Methotrexate-Anchored Yeast Three-Hybrid Screen.” ACS Chem. Biol., 8, 549-558 (2013).

68. "A common diaryl ether intermediate for the gram-scale synthesis of oxazine and xanthene fluorophores." Anzalone AV, Wang TY, Chen Z, Cornish VW. Angew Chem Int Ed Engl, 52(2):650-4 (2013). [pubmed] [full-text]


67. "A Heritable Recombination System for Synthetic Darwinian Evolution in Yeast." Romanini DW, Peralta-Yahya P, Mondol V, Cornish VW. ACS Synth Biol, 1(12):602–609 (2012). [full-text]

66. "Structural analysis of the Asn152Gly mutant of P99 cephalosporinase." Ruble JF, Lefurgy ST, Cornish VW, Powers RA. Acta Crystallogr D Biol Crystallogr, 68(Pt 9):1189-93 (2012). [pubmed] [full-text]

65. "Second-generation covalent TMP-tag for live cell imaging." Chen Z, Jing C, Gallagher SS, Sheetz MP, Cornish VW. J Am Chem Soc., 134(33):13692-9 (2012). [pubmed] [full-text]

64. "Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag." Gatzogiannis E, Chen Z, Wei L, Wombacher R, Kao YT, Yefremov G, Cornish VW, Min W. Chem Commun (Camb)., 48(69):8694-6 (2012). [pubmed] [full-text]

63. "Protein labelling: Playing tag with proteins." Romanini DW, Cornish VW. Nat Chem, 4(4):248-50 (2012). [pubmed] [full-text]


62. "Reiterative Recombination for the in vivo assembly of libraries of multigene pathways" Wingler LM, Cornish VW. PNAS, 108(37):15135-15140 (2011). [pubmed] [full-text]

61. "Chemical Tags for Labeling Proteins Inside Living Cells" Jing CR, Cornish VW. Acc Chem Res, 44(9):784-792 (2011). [pubmed] [full-text]

60. "Chemical tags: applications in live cell fluorescence imaging." Wombacher R, Cornish VW. J Biophotonics, 4(6):391-402 (2011). [pubmed] [full-text]

59. "A library approach for the discovery of customized yeast three-hybrid counter selections." Wingler LM, Cornish VW. Chembiochem., 12(5):715-7 (2011). [pubmed] [full-text]

58. "Ordered and dynamic assembly of single spliceosomes." Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ. Science, 331(6022):1289-95 (2011). [pubmed] [full-text]


57. "PCRless library mutagenesis via oligonucleotide recombination in yeast." Pirakitikulr N, Ostrov N, Peralta-Yahya P, Cornish VW. Protein Sci, 19(12):2336-46 (2010). [pubmed] [full-text]

56. "Live-cell super-resolution imaging with trimethoprim conjugates." R. Wombacher, M. Heidbreder, S. van de Linde, M.P. Sheetz, M. Heilemann, V.W. Cornish, M. Sauer. Nat Methods, 7, 717-9 (2010). [pubmed] [full-text]

55. "A trimethoprim-based chemical tag for live cell two-photon imaging." S.S. Gallagher, C. Jing, D.S. Peterka, M. Konate, R. Wombacher, L.J. Kaufman, R. Yuste, V.W. Cornish. Chembiochem, 11, 782-784 (2010). [full-text]

54. "Cytoskeletal coherence requires myosin-IIA contractility." Y. Cai, O. Rossier, N.C. Gauthier, N. Biais, M.-A. Fardin, X. Zhang, L.W. Miller, B. Ladoux, V.W. Cornish, M.P. Sheetz. J. Cell Sci., 123, 413-423 (2010). [pubmed] [full-text]


52. "Natural amino acids do not require their native tRNAs for efficient selection by the ribosome." P.R. Effraim, J. Wang, M.T.Englander, J. Avins, T.S. Leyh, R.L. Gonzalez Jr., V.W. Cornish. Nat. Chem. Biol., 5, 947-953 (2009). [full-text]

52. "Slow Peptide Bond Formation by Proline and Other N-alkylamino Acids in Translation." M. Pavlov, R. Watts, Z. Tan, V.W. Cornish, M. Ehrenberg, A. Forster. Proc. Natl. Acad. Sci., 106, 50-54 (2009). [PubMed]

51.  "Fluorescence in Living Systems: Applications in Chemical Biology." V.W. Cornish, S.S. Gallagher. In Wiley Encyclopedia of Chemical Biology, T.P. Begley, Editor. 2009, John Wiley & Sons, Inc.: Hoboken. pp. 28-38 (2009).

50. “An In Vivo Covalent TMP-tag Based on Proximity-Induced Reactivity.” S.S. Gallagher, J.E. Sable, M.P. Sheetz, V.W. Cornish.  ACS Chem. Biol., 4 547-556 (2009). [PubMed]


49. "Transcription Factor Logic Using Chemical Complementation." J.E. Bronson, W.W. Mazur, V.W. Cornish. Molecular BioSystems, 4, 56 (2008). [PubMed]

48.  "Characterization of a New Glycosynthase Cloned Using Chemical Complementation." H.Tao, P. Peralta-Yahya, J. Decatur, V.W. Cornish. ChemBioChem, 9, 681-684 (2008). [PubMed]

47. "Conditional Glycosylation in Eukaryotic Cells Using a Biocompatible Chemical Inducer of Dimerization." J.L. Czlapinski, M.W. Schelle, L.W. Miller, S.T. Laughlin, J.J. Kohler,  V.W. Cornish, C.R. Bertozzi.  J. Am. Chem. Soc., 130, 13186-13187 (2008). [PubMed]

46. "A High-Throughput Selection for Cellulase Catalysts Using Chemical Complementation." P. Peralta-Yahya, B. T. Carter, H. Lin, H. Tao. V.W. Cornish. J. Am. Chem. Soc., 130, 17446-17452 (2008). [PubMed]

45. "Chemical Biology Tools for Live Cell Imaging." S.S. Gallagher, V.W. Cornish. In Encyclopedia for Chemical Biology, Germany: Wiley-VCH Verlag GmbH & Co., in press. (Invited Review).


44. "Optimized Fluorescent Trimethoprim Derivatives for In Vivo Protein Labeling." N.T. Calloway, M. Choob, A. Sanz, M.P. Sheetz, L.W. Miller, V.W. Cornish. ChemBioChem., 8, 767-774 (2007). [PubMed]

43. "Specificity of Translation for N-Alkyl Amino Acids." B. Zhang, Z. Tan, L.G. Dickson, M.N.L. Nalam, V.W. Cornish, A. Forster. J. Am. Chem. Soc., 129, 11316-11317 (2007). [PubMed]

42. "Saturation Mutagenesis of Asn152 Reveals a Substrate Selectivity Switch in P99 Cephalosporinase." S.T. Lefurgy, R.M. de Jong, V.W. Cornish. Protein Science, 16, 2636-2646, (2007).[PubMed]

41. "An Orthogonal Dexamethasone-Trimethoprim Yeast Three-Hybrid System." S.S. Gallagher, L.W. Miller, V.W. Cornish. Anal. Biochem., 363, 160-162, (2007). [PubMed]


40. "Optimized Design and Synthesis of Chemical Dimerizer Substrates for Detection of Glycosynthase Activity via Chemical Complementation." H. Tao, P. Peralta-Yahya, H. Lin, V.W. Cornish. Bioorg. Med. Chem., 14, 6940-6953 (2006). [PubMed]

39. "Bringing the Power of Genetics to Chemistry." P. Peralta-Yahya, V.W. Cornish. In Chemical Biology, S.L. Schreiber, T. Kapoor, G. Weiss, Eds. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, vol. 1, pp. 199-226 (2006). (Invited Review).

38. "Biological Chemistry: Catalytic Competition for Cells.” V.W. Cornish. Nature, 440, 156-157 (2006). (Invited News and Views). [PubMed]

37. "Chemical Complementation." S. Lefurgy, V.W. Cornish. In Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting, J-L. Reymond, Ed. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, pp. 183-219 (2006). (Invited Review).


36. "De Novo Genetic Codes and Pure Translation Display." Z. Tan, S.C. Blacklow, V.W. Cornish, A.C. Forster. Methods, 36, 279-90 (2005). [PubMed]

35. "Investigation of the Mechanism of Resistance to Third-Generation Cephalosporins by Class C b-Lactamases Using Chemical Complementation." B.T. Carter, H. Lin, S.D. Goldberg, E.A. Althoff, J. Raushel, V.W. Cornish. ChemBioChem, 6, 2055-2067 (2005). [PubMed]

34. "In Vivo Labeling with Trimethoprim Conjugates: A Flexible Chemical Tag." L. Miller, Y. Cai, M. Sheetz, V.W. Cornish. Nature Methods, 2, 255-257 (2005). [PubMed]

33. "Selective Chemical Labeling of Proteins in Living Cells." L. Miller, V.W. Cornish. Curr. Opin. Chem. Biol., 9, 1-6 (2005). (Invited Review). [PubMed]


32. "Pure Translation Display," A.C. Forster, V.W. Cornish, S.C. Blacklow. Anal. Biochem., 333, 358-364 (2004). [abstract] [pdf]

31. "Amino acid backbone specificity of the Escherichia coli translation machinery,"  Z. Tan, A.C. Forster, S.C. Blacklow, V.W. Cornish. J. Am. Chem. Soc., 126, 12752-12753 (2004). [abstract] [pdf]

30. "Correlation between ligand-receptor affinity and the transcription readout in a yeast three-hybrid system," K.S. de Felipe, B.T. Carter, E.A. Althoff, V.W. Cornish. Biochemistry, 43, 10353-10363. [abstract] [pdf]

29. "Correlation Between Catalytic Efficiency and the Transcription Read-Out in Chemical Complementation, a High-Throughput Assay for Enzyme Catalysis." D. Sengupta, H. Lin, S. Goldberg, J. Mahal, V.W. Cornish. Biochemistry, 43, 3570-3581 (2004).[abstract] [pdf]

28. "Directed Evolution of a Glycosynthase Via Chemical Complementation." H. Lin, H. Tao, V.W. Cornish. J. Am. Chem. Soc., 126, 15051-15059 (2004). [abstract] [pdf]

27. "Correlation Between Ligand-Receptor Affinity and the Transcription Readout in a Yeast Three-Hybrid System." K. Suwwan deFelipe, E.A. Althoff, B.T. Carter, V.W. Cornish. Biochemistry, 43, 10353-10363 (2004).

26. "Finding Cinderella After the Ball: a Three-Hybrid Approach to Drug Target Identification." S. Lefurgy, V.W. Cornish. Chem. Biol., 11, 151-153 (2004). (Invited Commentary).

25. "Correlation Between Catalytic Efficiency and the Transcription Read-Out in Chemical Complementation, a High-Throughput Assay for Enzyme Catalysis." D. Sengupta, H. Lin, S. Goldberg, J. Mahal, V.W. Cornish. Biochemistry, 43, 3570-3581 (2004).

24. "Mixed Quantum Mechanical/Molecular Mechanical (QM/MM) Study of the Deacylation Reaction in a Penicillin Binding Protein (PBP) Versus in a Class C b-Lactamase," B.F. Gherman, S.D. Goldberg, V.W. Cornish, R.A. Friesner. J. Am. Chem. Soc., 126, 7652-7664 (2004).[abstract] [pdf]

23. "Methotrexate Conjugates: A Modular In Vivo Protein Tag," L. Miller, J. Sable, P. Goelet, M. Sheetz, V.W. Cornish. Angew. Chem. Int. Ed. Engl., 43, 1672-1675 (2004). [abstract] [pdf]


22. "Identification of Residues Critical for Catalysis in a Class C b-Lactamase," S. Goldberg, W. Iannuccilli, T. Nguyen, J. Ju, V.W. Cornish. Protein Science, 12, 1633-1645 (2003). [full-text] [pdf]

21. "Programming peptidomimetic syntheses by translating genetic codes designed de novo," A. Forster, Z. Tan, M.N.L. Nalam, H. Lin, H. Qu, V.W. Cornish, S. Blacklow. Proc. Natl. Acad. Sci. USA, 100, 6353-6357 (2003). [full-text] [pdf]

20. "An Optimized Dexamethasone-Methotrexate Yeast 3-Hybrid System for High-Throughput Screening of Small Molecule-Protein Interactions," K. Baker, D. Sengupta, G. Salazar-Jimenez, V.W. Cornish. Anal. Biochem., 315, 134-137 (2003). [full-text] [pdf]


19. "Chemical Complementation: A Reaction-Independent High-Throughput Genetic Assay for Enzyme Catalysis," K. Baker*, C. Bleczinski*, G. Salazar-Jimenez, H. Lin, D. Sengupta, S. Krane, V.W. Cornish. Proc. Natl. Acad. Sci. USA, 99, 16537-16542 (2002). [abstract] [full-text] [pdf]
Featured in a commentary in Proc. Natl. Acad. Sci. USA, 99, 16513-16515 (2002) [full-text] [pdf] and in Chem. & Eng. News, 81, 24 (2003). [full-text]

18. "Milestones in Directed Enzyme Evolution," H. Tao, V.W. Cornish. Curr. Opin. Chem. & Biol., 6, 858-864 (2002). [abstract] [pdf]

17. Receptor-Dependence of the Transcription Read-Out in a Small-Molecule Three-Hybrid System, W. Abida, B. Carter, E. Althoff, H. Lin, V.W. Cornish, ChemBioChem, 3, 887-895 (2002). [abstract] [pdf]

16. "A Bacterial Small-Molecule 'Three-Hybrid' System," E.A. Althoff, V.W. Cornish, Angew. Chem., 114, 2433-2436 (2002); Angew. Chem. Int. Ed., 41, 2327-2330 (2002). [abstract] [pdf]

15. "Screening and Selection Methods for the Large Scale Analysis of Protein Function," H. Lin, V.W. Cornish, Angew. Chem., 114, 4580-4606 (2002); Angew. Chem. Int. Ed., 41, 4402-4425 (2002). [abstract] [pdf]

14. "Yeast n-Hybrid Systems for Molecular Evolution," B.T. Carter, H. Lin, V.W. Cornish, in Directed Molecular Evolution of Proteins, S. Brakmann, K. Johnsson, Eds. Wiley-VCH Verlag, Weinheim. ISBN 3-527-30423-1 [publisher]

13. "Probing Enzyme Complexity," V.W. Cornish, Chem. & Eng. News, 79, 64 (2001). Young Investigator Featured in 125th Year Anniversary Issue. [pdf]


12. "In Vivo Protein-Protein Interaction Assays: Beyond Proteins," H. Lin, V. W. Cornish, Angew. Chem. Int. Ed. Engl., 40, 871-875 (2001) [abstract] [pdf]


11. "Dexamethasone-Methotrexate: An Efficient Chemical Inducer of Protein Dimerization In Vivo," H. Lin, W. Abida, R.T. Sauer, V.W. Cornish, J. Am. Chem. Soc., 122, 4247-4248 (2000). [description] [pdf]


10. "Photoaffinity Labeling and Mass Spectrometry Identify Ribosomal Protein S3 as a Potential Target for Hybrid Polar Cytodifferentiation Agents," Y. Webb, X. Zhou, L. Ngo, V.W. Cornish, J. Stahl, H. Erdjument-Bromage, P. Tempst, R. Rifkind, P. Marks, R. Breslow and V. Richon, J. Biol. Chem., 274, 14280-14287 (1999). [abstract] [pdf]

9. "Insertion of Unnatural Amino Acids Using Chemical Acylation Techniques," J.S. Thorson, V.W. Cornish, J.E. Barrett, S.T. Cload, T. Yano, P.G. Schultz, Methods in Molecular Biology: Protein Synthesis: Methods and Protocols, 77, 43-73 (1998).

8. "An Experimental Approach to Evaluating the Role of Backbone Interactions in Proteins Using Unnatural Amino Acid Mutagenesis," J.T. Koh, V.W. Cornish, P.G. Schultz, Biochemistry, 36, 11314-11322 (1997). [abstract] [pdf]

7. "Site-Specific Protein Modification Using a Ketone Handle," V.W. Cornish, K.M. Hahn, P.G. Schultz, J. Am. Chem. Soc., 118, 8150-8151 (1996). [full-text] [pdf]

6. "Probing Protein Structure and Function with an Expanded Genetic Code," V.W. Cornish, D. Mendel, P.G. Schultz, Angew. Chem. Int. Ed. Engl., 34, 621-633 (1995). [abstract] [pdf]

5. "Site-Directed Mutagenesis with an Expanded Genetic Code," D. Mendel, V.W. Cornish, P.G. Schultz, Ann. Rev. Biophys. Biomol. Struct., 24, 435-462 (1995). [full-text]

4. "Stabilizing and Destabilizing Effects of Placing b-Branched Amino Acids in Protein a-Helices," V.W. Cornish, M.I. Kaplan, D.L. Veenstra, P.A. Kollman, P.G. Schultz, Biochemistry, 33, 12022-12031 (1994). [abstract] [pdf]

3. "A New Tool for Studying Protein Structure and Function," V.W. Cornish, P.G. Schultz, Curr. Opin. Struct. Biol., 4, 601-607 (1994).

2. "Site-Specific Incorporation of Biophysical Probes into Proteins," V.W. Cornish, D.R. Benson, C.A. Altenbach, K. Hideg, W.L. Hubbel, P.G. Schultz, Proc. Natl. Acad. Sci. USA, 91, 2910-2914 (1994). [abstract] [pdf]

1. "Probing the Role of Loop 2 in Ras Function with Unnatural Amino Acids," H.H. Chung, D.R. Benson, V.W. Cornish, P.G. Schultz, Proc. Natl. Acad. Sci. USA, 90, 10145-10149 (1993). [abstract] [pdf]