Demonstrations & Passwords |
User Guides to Experiments |
Videos |
All new users must have an introductory demonstration by one of the NMR staff. This introductory Bruker demo is needed for access to the Bruker 300NB, 300WB, and 400. Access to the 500 requires further training (see below).
An account and password are required to use the Bruker instruments. After you have taken the intro. demo, follow this procedure (only for the Bruker NMRs):
1. On one of the data stations not attached to an NMR, learn the processing and plotting software. Practice at least once. Use the login-name "practice". The password in posted next to the computer. You may practice on the data stations at any time.
2. On the 300wb NMR, sign up and practice. One of the NMR staff must be there to log you in. Sign-up for time when they are here (9:30 AM-6 PM).
3. When you have learned everything on the introductory handout, make arrangements with the staff to watch you take and process a spectrum, and if you pass, give you a password.
This is an optional demonstration that covers the following topics: tuning the probe, controlling the decoupler with different pulse programs, parameters to enhance quaternary carbons, and DEPT spectra. This is recommended for all NMR users. A sign-up sheet is posted in room 208 Havemeyer and demos are given when 4 to 5 people have signed-up.
This optional demo covers instructions for the 2D NOESY experiment and for the magnitude COSY experiment. A sign-up sheet is posted in room 208 Havemeyer and demos are given when 4 to 5 people have signed-up.
People who desire to be 500 users must first attend the COSY and NOESY demo and reproduce the results of that demo. Training on the 500 is by arrangement.
Demos such as HSQC, HMBC, TOCSY, kinetics, water-suppression, variable-temperature, T1 measurement, magnetization transfer, etc..., are given on an as-needed basis.
The following are step-by-step guides to NMR experiments on Bruker NMRs. These are customized for use here only at Columbia. There are in Adobe pdf format:
One Dimension Proton NMR using Bruker NMR Instruments
COSY Spectra and Homonuclear Decoupling
Carbon and Heteronuclear One Dimension Spectra
NMR processing /w MestreNova on PCs in your lab - DATA SERVER
NMR spectral prediction using MestReNova