Contact

Education

2003–2007

PhD in molecular biophysics, Paris 6 University (Pierre & Marie Curie), France.
PhD defended and obtained on March 27th, 2007.
Expertise: cryoelectron microscopy, single-particle image processing and 3D reconstructions, molecular modelling, fitting of atomic data.

2002–2003

M.Sc. in Bioinformatics ("Sequence analysis et molecular modelling") – Paris 7 University, France. M.Sc. obtained in July 2003.

2001–2002

B.Sc. in biochemistry - Toulouse 3 University, France. B.Sc. obtained in July 2002.

Research experience

2007-present

Research Affiliate, HHMI, department of Computational Biology and Molecular Imaging.

2003–2007

PhD, IMPMC (Institut de Minéralogie et de physique des Milieux Condensés), at Paris 6 laboratory). Subject: Structural studies of multienzymatic complexes by cryoelectron microcopy, image analysis, 3D reconstruction, homology modelling, and fitting of atomic data into density maps, under the direction of Nicolas Boisset.

2003

6 months M.Sc. internship, CEA (Commissariat à l’Énergie Atomique), Saclay (FR), in the Biology Department (Département de Biologie Joliot-Curie), under the direction of François André and Marcel Delaforge.
Subject: Structure and metabolism of cytochromes P450s 3A: molecular modelling and docking simulations of substrates into active sites of cytochromes P450s.

2001–2002

4 months B.Sc. internship, INRA center of Toulouse (FR), xenobiotic laboratory (laboratoire des Xénobiotiques), under the direction of Françoise Guéraud.
Subject: impact of growth hormon on hepatic antioxidant defense mechanisms in rat cells. RNA modifications studies (semi-quantitative RT-PCR), disturbance of enzymatic activities (western blotting, activity measures).

2001

Summer internship, MilleGen Society (Toulouse, FR). Classic techniques in molecular biology (bacterial harvest, DNA extraction, restriction enzymes, electrophoresis); computer processing of sequencing data.

Skills

Image processing

Single-particle image processing and 3D reconstructions.

Bioinformatics

Sequence analysis of proteins and secondary structure predictions.
Homology modelling, simulations of molecular dynamics.
Refinement of atomic data in cryoEM density maps.

Computer skills

OS: Unix, Linux, Windows.
Languages: C, awk, HTML, notions in Python.

Languages

French (mother tongue), English, notions in German.

Research interests

Cryoelectron microscopy, single-particle image processing, 3D reconstructions of macromolecules, homology modelling, rigid-body and flexible fitting of atomic data into cryoEM density maps.

Teaching experience

2006

Teaching assistant at Paris 6 University and examination of M.Sc. students.

2006

Supervision of M.Sc. students in IMPMC, on image analysis and 3D reconstruction.

Publications

Journals

Cottevieille, M., Larquet, E., Jonic, S., Petoukhov, M. V., Caprini, G., Paravisi, S., Svergun, D. I., Vanoni, M. A. and Boisset, N. (2008). The Subnanometer Resolution Structure of the Glutamate Synthase 1.2-MDa Hexamer by Cryoelectron Microscopy and Its Oligomerization Behavior in Solution: FUNCTIONAL IMPLICATIONS. J Biol Chem 283, 8237-49.

Busselez*, J., Cottevieille*, M., Cuniasse, P., Gubellini, F., Boisset, N., and Levy, D. (2007). Structural basis for the PufX-mediated dimerization of bacterial photosynthetic core complexes. Structure 15, 1674-83.
* authors contributed equally.

Jonić, S., Sorzano, C.O., Cottevieille, M., Larquet, E., and Boisset, N. 2007. A novel method for improvement of visualization of power spectra for sorting cryo-electron micrographs and their local areas. J Struct Biol 157: 156-167.

Sorzano, C.O., Jonic, S., Cottevieille, M., Larquet, E., Boisset, N., and Marco, S. 2007. 3D electron microscopy of biological nanomachines: principles and applications. Eur Biophys J 36(8): 995-1013.