vectors
pBI series of Drosophila vectors
Ji-Wu Wang and Brian McCabe, manuscript in preparation
pBI (AttB, Insulated) Drosophila Transgenic Vectors are based on a PhiC31 compatible backbone with miniwhite marker and ampicillin resistance (Groth et al. 2004, Dietzl et al. 2007). Inserts are flanked by gypsy insulator elements to increase expression levels and reduce insertion site-to-site expression variability (Markstein et al. 2008). GAL4 versions of these vectors combine 10 copies of the Gal4 Upstream Activating Sequence (UAS) to improve expression levels (Dietzl et al. 2007) with a Drosophila synthetic core promoter (DSCP) to reduce non-specific tissue expression (Pfeiffer et al. 2008), which together we call UASC. Most vectors also utilize gateway technology to facilitate cloning.
For full sequences and maps, click on the links in the right sidebar.
pBI series of Drosophila vectors
Ji-Wu Wang and Brian McCabe, manuscript in preparation
pBI (AttB, Insulated) Drosophila Transgenic Vectors are based on a PhiC31 compatible backbone with miniwhite marker and ampicillin resistance (Groth et al. 2004, Dietzl et al. 2007). Inserts are flanked by gypsy insulator elements to increase expression levels and reduce insertion site-to-site expression variability (Markstein et al. 2008). GAL4 versions of these vectors combine 10 copies of the Gal4 Upstream Activating Sequence (UAS) to improve expression levels (Dietzl et al. 2007) with a Drosophila synthetic core promoter (DSCP) to reduce non-specific tissue expression (Pfeiffer et al. 2008), which together we call UASC. Most vectors also utilize gateway technology to facilitate cloning.
For full sequences and maps, click on the links in the right sidebar.
|
NAME
|
PURPOSE
|
NOTES
|
|
|
|
|---|---|---|---|---|---|
|
pBI
|
Transgenic DNA
fragments, e.g. Genomic fragments
|
Similar
restrictions sites to pUAST
|
|
|
|
|
pBI-G
|
Transgenic
DNA fragments, e.g. Genomic
fragments
|
Gateway
compatible
|
|
|
|
|
pBI-UASC
|
Gal4 dependent
gene expression
|
Similar
restrictions sites to pUAST.
|
|
|
|
|
pBI-UASC-G
|
Gal4 dependent
gene expression
|
Gateway compatible
|
|
|
|
|
pBI-UASC-VG
|
Gal4 dependent gene expression, mVenus
(YFP) fusion
|
Gateway compatible, Venus fused to the
N-terminus of the inserted gene
|
|
|
|
|
pBI-UASC-GV
|
Gal4 dependent gene expression, mVenus
(YFP) fusion
|
Gateway compatible, Venus fused to the
C-terminus of the inserted gene
|
|
|
|
|
pBI-UASC-GGi.
|
Gal4 dependent gene expression, transgenic
RNA inhibition (RNAi)
|
Gateway compatible, 2 inverted gateway
cassettes separated by ftz intron
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|