Ji-Wu Wang and Brian McCabe, manuscript in preparation
pBI (AttB, Insulated) Drosophila Transgenic Vectors are based on a PhiC31 compatible backbone with miniwhite marker and ampicillin resistance (Groth et al. 2004, Dietzl et al. 2007). Inserts are flanked by gypsy insulator elements to increase expression levels and reduce insertion site-to-site expression variability (Markstein et al. 2008). GAL4 versions of these vectors combine 10 copies of the Gal4 Upstream Activating Sequence (UAS) to improve expression levels (Dietzl et al. 2007) with a Drosophila synthetic core promoter (DSCP) to reduce non-specific tissue expression (Pfeiffer et al. 2008), which together we call UASC. Most vectors also utilize gateway technology to facilitate cloning.
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|
Name |
Purpose |
Features |
|
pBI |
Transgenic DNA fragments, e.g. Genomic fragments |
Similar restrictions sites to pUAST |
|
pBI-G |
Transgenic DNA fragments, e.g. Genomic fragments |
Gateway compatible |
|
pBI-UASC |
Gal4 dependent gene expression |
Similar restrictions sites to pUAST |
|
pBI-UASC-G |
Gal4 dependent gene expression |
Gateway compatible |
|
pBI-UASC-VG |
Gal4 dependent gene expression, mVenus (YFP) fusion |
Gateway compatible, Venus fused to the N-terminus of the inserted gene |
|
pBI-UASC-GV |
Gal4 dependent gene expression, mVenus (YFP) fusion |
Gateway compatible, Venus fused to the C-terminus of the inserted gene |
|
pBI-UASC-GGi |
Gal4 dependent gene expression, transgenic RNA inhibition (RNAi) |
Gateway compatible, 2 inverted gateway cassettes separated by ftz intron |