• DNA Vectors

    pBID series of Drosophila vectors

    Ji-Wu Wang, Erin Beck and Brian McCabe, (2012) PLoS ONE 7(7): e42102

    pBID
    pBID (AttB, Insulated, Drosophila) transgenic vectors are based on a PhiC31 compatible backbone with a miniwhite marker and ampicillin resistance. Inserts are flanked by gypsy insulator elements to reduce insertion site-to-site expression variability. GAL4 compatible versions of these vectors combine 10 copies of the Gal4 Upstream Activating Sequence (UAS) with a Drosophila synthetic core promoter (DSCP) to reduce non-specific tissue expression which together we call UASC. Most vectors also utilize gateway technology to facilitate high-throughput cloning.

    For details of the construction and evaluation of these vectors see
    Wang et al. 2012.

    A zip file with data sheets, full sequences and maps for all the vectors is available for download
    here.

    Maps are in
    ApE format.

    All vectors are available from Addgene -
    here

    pMartini G series of Gateway vectors

    Ji-Wu Wang, Erin Beck and Brian McCabe, (2012) PLoS ONE 7(7): e42102

    The pMartini G series of vectors were constructed to allow easy conversion of vectors to gateway destination vectors and to facilitate the assembly of new fusion vectors that are gateway compatible. Each vector has a single orientation of one of three Gateway cassettes surrounded by multiple cloning sites (6 vectors total).

    Details of the construction of these vectors is available in
    Wang et al. 2012.

    A zip file with data sheets, full sequences and maps for all the vectors is available for download
    here.

    Maps are in
    ApE format.

    All vectors are available from Addgene -
    here

    Other Constructs

    To request other DNA constructs please follow the instructions for fly stocks.
  • Fly Stocks
    To request Drosophila stocks (or DNA constructs not in Addgene) from any of our publications -

    email Tim (ordersATmccabelab.org) with

    your name,
    your lab,
    full address,
    phone number (required for Fedex)
    and Fedex account number.