THE CONDUCT OF CLINICAL TRIALS OF MATERNAL-NFANT TRANSMISSION OF HIV

SUPPORTED BY THE UNITED STATES DEPARTMENT OF

HEALTH AND HUMAN SERVICES IN DEVELOPING COUNTRIES

A SUMMARY OF THE NEEDS OF DEVELOPING COUNTRIES,

THE SCIENTIFIC APPLICATIONS, AND THE ETHICAL CONSIDERATIONS

ASSESSED BY THE NATIONAL INSTITUTES OF HEALTH AND

THE CENTERS FOR DISEASE CONTROL AND PREVENTION

1994-1997

JULY 1997

For the past three years the United States Department of Health and

Human Services (HHS), through its National Institutes of Health (NIH)

and Centers for Disease Control and Prevention (CDC), has been engaged

in the development and conduct of clinical trials designed to identify

feasible interventions for preventing maternal-infant transmission of

HIV in developing countries. The Director, NIH, the Director, CDC, and

other senior scientists and administrators within the NIH and CDC, at

the request of the Secretary, HHS, conducted a thorough assessment of

the previous reviews of the needs, resources, and health care

capacities of these developing countries and the process of scientific

development and ethical evaluation leading up to and guiding the

current conduct of these clinical trials. As an added measure,

comments of a number of experts in biomedical ethics and the

biosciences outside of NIH and CDC were also sought and considered.

Based on this assessment, NIH and CDC have determined that, although

these are complex matters, the studies have the potential to be of

enormous value to the developing countries and are scientifically

well-founded and ethically acceptable.

The NIH/CDC assessment addressed three major questions related to

these clinical trials. What is the need for these studies? Are the

studies adequately designed to examine options for treatment that will

meet that need? Is the involvement of human subjects in these studies

consistent with the internationally accepted principles of autonomy,

justice, and beneficence as implemented by the United States Federal

Policy for the Protection of Human Subjects, including the equitable

selection of subjects, obtaining of voluntary informed consent, and

employing the input, review, and authorization of the appropriate

ethical and other bodies in the U.S. and in the developing countries

where the research is being conducted?

THE NEED

One regimen of antiretroviral therapy has been shown to reduce

substantially the likelihood of maternal-infant transmission of HIV.

The identification of this successful regimen was the result of the

National Institutes of Health's AIDS Clinical Trials Group protocol

076 (ACTG 076 or 076) in 1994. In spite of this knowledge,

approximately 1,000 HIV-infected infants are born each day, the vast

majority of them in developing countries. This occurs, in part,

because the regimen proven to be effective is simply not feasible as a

standard of prevention in much of the developing world.

There are two reasons for this lack of feasibility. First, to follow

the regimen that has proven efficacy requires that the women be

reached early in prenatal care; be tested for and counseled concerning

their HIV status; comply with a lengthy oral treatment regimen;

receive intravenous administration of the antiretroviral zidovudine

(ZDV or AZT) during labor and delivery; and refrain from

breast-feeding. Additionally, the newborns must receive 6 weeks of

oral AZT therapy. During and after the time the mother and infant are

treated with AZT, both must be carefully monitored for adverse effects

of exposure to this drug. In the developing world countries that are

the sites of these studies, these requirements could seldom be

achieved, even under the infrequent circumstance when women present

early enough for the screening and care requirements of the 076

therapeutic regimen to be implemented. Second, the wholesale drug

costs for the AZT in the 076 regimen are estimated to be in excess of

$800, an amount far greater than these developing countries could

afford as standard care. For example, in the developing country of

Malawi, the cost of AZT alone for the 076 regimen for one HIV-infected

pregnant woman and her child is more than 600 times the annual health

care budget allocation for one person. Less complex and expensive

alternatives are urgently needed to address the staggering impact of

maternal-infant transmission of HIV in developing countries.

In June 1994, after the results of ACTG 076 were released, the World

Health Organization (WHO) convened a group of researchers and public

health practitioners from around the world in Geneva. This

international panel called for the use of the 076 regimen in the

industrialized world, where it is feasible, but immediately called for

the exploration of alternative regimens that could be used in the

developing world, stating that logistical issues and cost would

preclude the widespread application of the 076 regimen. The WHO panel

called for international coordination of research efforts to develop

simpler, less costly drug regimens. This coordination continues in the

form of meetings two to three times per year of the UNAIDS Informal

Working Group on Prevention of Mother to Child Transmission of HIV. As

a result, there is a global research agenda addressing the need to

devise efficacious regimens that can be safely and widely implemented

in the developing world.

THE STUDY DESIGNS

The NIH- and CDC-supported studies of maternal-infant transmission of

HIV in developing countries are designed to meet the critical need

just described. The panel convened by WHO in Geneva stated in

Recommendation 6 of its Recommendations from the Meeting on Prevention

of Mother-to-Infant Transmission of HIV by Use of Antiretrovirals,

Geneva 23-25 June 1994 (attached as an appendix to this report):

Since the ZDV regimen studied in ACTG 076 is not applicable

in those parts of the world where most MTI

[mother-to-infant] transmission of HIV occurs,

placebo-controlled trials offer the best option for

obtaining rapid and scientifically valid results.

The WHO panel went on to explain in its commentary on the

recommendation:

Most of MTI transmission of HIV occurs in the developing

world, where the ZDV regimen used in ACTG 076 is not

applicable because of its cost and operational requirements.

In those parts of the world, the choice of a placebo for the

control group of a randomized trial would be appropriate as

there is currently no effective alternative for HIV-infected

pregnant women.

For each individual study there has been careful consideration of the

specific needs of and treatment feasibility within the country in

which it would be implemented. NIH, CDC, collaborating U.S.

institutions, and the host countries will continue to monitor each

study and any changes in the countries that may have an impact on

study design. It is an unfortunate fact that the current standard of

perinatal care for the HIV-infected pregnant women in the sites of the

studies does not include any HIV prophylactic intervention at all. Nor

does the standard of care for these HIV-infected women include the

combination therapies recommended and used for some HIV-infected women

in the U.S. However, the inclusion of this regrettable, but real,

performance-site standard in the form of placebo controls provides the

direct comparison of standard and new intervention that is needed to

form the basis for rational policy decisions and will result in the

most rapid, accurate, and reliable answer to the question of the value

of the intervention being studied compared to the local standard of

care.

The NIH- and CDC-supported studies are designed in a manner consistent

with the still-pertinent recommendations of the WHO panel and have

been developed in on-site collaboration with the health ministries,

physicians, and researchers of the host countries. There is strong

support for the design of the NIH- and CDC-supported studies,

including the placebo arms, within the countries where the clinical

trials are being carried out, in part because the studies are

assessing regimens that might realistically be employed to decrease

mother-to-infant transmission, taking into account the health care

resources and operational capabilities of these countries.

HUMAN RESEARCH SUBJECT PROTECTIONS

The acceptability of the involvement of human subjects in the studies

under discussion has been scrupulously reviewed beginning with the

first proposal for ACTG 076 which was a placebo-controlled study

conducted in the U.S. and France. After the complex 076 treatment

regimen was proven successful, the WHO conference in Geneva quickly

issued clear guidelines for research taking into account the extremely

wide range of health care capabilities that characterize the broad

spectrum of countries described by the term developing countries. As

mentioned earlier, these matters have been debated in formal

discussions, forums, and required reviews in the U.S., in

international settings, and in the countries where the clinical trials

are or will be carried out.(1) Even so, CDC determined that Assurances

for performance site countries required by HHS regulations at Title

45, Part 46, Section 103 had not been obtained prior to enrolling

subjects. CDC acted immediately to notify OPRR and to address these

administrative procedures. The Assurances for the CDC-Thailand and

CDC-Cote d'Ivoire studies were conditionally approved by OPRR on July

7, 1997 and July 14, 1997 respectively. CDC amended its human subjects

protection administrative procedures to be in full compliance with 45

CFR 46.103.

Arguments against the NIH- and CDC-supported studies appear to rest on

the proposition that it is unethical to conduct a clinical trial

unless it offers all participants a chance to receive an effective

intervention if such is available anywhere in the world, even if it is

not available at the site of the clinical trial. Ideally, this would

be so for all clinical trials for all therapies. But the reality is

that often it is not possible. The very purpose of the NIH- and

CDC-supported studies of maternal-infant transmission of HIV in

developing countries is to identify interventions other than those of

076 and we agree with the WHO Geneva panel's Recommendation 2 that:

It should be emphasized that the results of ACTG 076 are

only directly applicable to a specific population. Moreover,

the ZDV regimen employed in the ACTG 076 study has a number

of features (cost, logistical issues, among others) which

limit its general applicability. Therefore, no global

recommendations regarding use of ZDV to prevent MTI

transmission of HIV can be made.

The use of historical controls was considered and found to be

unacceptable because existing epidemiological data for the host

countries are inadequate for this purpose. We agree with the

commentary on Recommendation 5 of the WHO Geneva panel that:

Randomized controlled trials offer the best evaluation of

new treatment regimens. The use of historical controls is

strongly discouraged, due to the wide changes with time in

the study population (differences in the distribution of

disease stages), the circulating viral strains, the

diagnostic tools used for the ascertainment of the HIV

infection status of infants (antibodies in earlier cohorts

compared to direct viral markers in more recent cohorts),

and the treatment practices (increased use of zidovudine in

HIV-infected pregnant women with AIDS-related symptoms

and/or CD4+ lymphocyte counts).

The WHO guidelines clearly indicate that the in-country health care

capabilities of each country in which maternal-infant HIV transmission

research is to be conducted must be used to define the type of

research which is ethical and therefore permissible in that country.

If a country will be able to afford only very minimal increments in

the resources directed toward improved perinatal care for HIV-infected

pregnant women and their children, then trials like those focused on

vitamin A and other micronutrients are ethical, permissible, and

desirable.

In other countries, such as Thailand, the situation is far more

complex. Thailand is a country that may be able to afford a simplified

AZT/076-like regimen. However, Thai researchers, physicians, and

public health officials are understandably interested in seeing a

number of issues addressed before any AZT is placed in general use in

HIV-infected pregnant women in their country: (1) Is a much simplified

076-like regimen (e.g., initiation of prenatal treatment at 36 weeks

of pregnancy, oral instead of intravenous AZT during labor and

delivery, elimination of AZT given to the infant) effective in

reducing transmission? (2) What adverse outcomes related to AZT use

will be seen in Thai populations? Will adverse effects not seen in the

U.S. and Europe occur in Thailand? (3) Is AZT administered orally to

Thai women safe and well-tolerated? Do Thai women metabolize AZT such

that the dose used is the right one to use in all Thai HIV-infected

pregnant women? and, (4) How does the type of HIV seen in Thailand

(which has demonstrable differences from the type seen in the U.S.)

respond to AZT?

Seeking answers to these questions are two studies, one supported by

CDC and one supported by NIH. The CDC-Thai study will determine how

well tolerated and how effective a very simple AZT regimen is in a

population of women who are infected with a subtype of HIV

predominantly different from that observed in the U.S. and who also

have co-factors for transmission that may differ from those seen in

American populations. Because it is a two-arm, placebo-controlled

clinical trial, the CDC-Thai study will provide rapid answers to many

of the important questions noted above. It also will enable the

Ministry of Health and physicians in Thailand to make better-informed

decisions about the use of a much-simplified AZT regimen for general

use in HIV-infected pregnant women.

Complementing the CDC-Thai study is an NIH-Thai study to determine how

much additional benefit, as compared to how much additional cost and

adverse effect, is occasioned by small increments in treatment

complexity. The NIH-Thai study has four arms, each a modest increment

over the treatment arm in the CDC-Thai study. However, even the most

complex arm of the NIH-Thai study is not identical to the treatment

arm of 076. The NIH-Thai study will benefit from the baseline that is

established by the CDC-Thai study.

Since the NIH-Thai study provides some level of AZT to all

participants, the data from the CDC-Thai study would be particularly

important for interpretation of results if the outcomes were the same

for each of the four arms. Taken together, these two studies will

provide a broad range of information about the likely value of AZT as

a strategy to interrupt maternal-infant transmission of HIV.

In an analysis of the spectrum of studies supported by the NIH and CDC

in Africa and Asia, it is clear that we have adhered to international

guidelines, including the recent WHO guidelines which address

specifically the ethical conduct of research in the post-076 era. The

primary consideration in decisions about the appropriate conduct of

research has been this: Once the research is completed at a given

site, will the population which the study participants represent be

able to profit from what is learned from that research?

The International Ethical Guidelines for Biomedical Research Involving

Human Subjects that were prepared by the Council for International

Organizations of Medical Sciences (CIOMS) in collaboration with WHO

are:

...intended to indicate how the ethical principles embodied

in the Declaration [of Helsinki] could be effectively

applied in developing countries.

To evaluate interventions that they could not implement realistically

would be exploitive of those in the participant country since there

would be no likelihood of meeting requirement 15 of the Guidelines

that obliges:

...any product developed [through] such research will be

made reasonably available to the inhabitants of the host

community or country at the completion of successful

testing...

Therefore, we have determined that the more compelling ethical

argument is against using a regimen that if found to be superior in

the study could not possibly be used in the prevention of

maternal-infant transmission of HIV in the host country. Turning once

again to Malawi for example, health officials there refused to permit

the conduct of a study involving a full course regimen of AZT (such as

that used in ACTG 076) because they believed it would be unethical to

undertake such a study in Malawi given that its very limited resources

and poor health infrastructure make the introduction of AZT as

standard treatment for HIV-infected pregnant women unfeasible.

Instead, the health officials wanted research on alternative treatment

approaches that might reduce maternal-infant transmission of HIV. The

justification and ethical foundation for the NIH- and CDC-supported

studies incorporate the reality that the clinical trials are examining

other alternatives that could actually be used for the majority of

HIV-infected pregnant women and mothers in the countries in which the

clinical trials are being carried out. The process of ethical review

of these trials has been rigorous. It has included community and

scientific participation and the application of the U.S. rules for the

protection of human research subjects in reviews by the relevant

institutional review boards (IRBs) in the U.S. and in the countries

where the clinical trials are carried out. Support from local

governments has been obtained and each active study has been and will

continue to be reviewed by an independent Data and Safety Monitoring

Board. These studies are in compliance with broadly accepted

principles of ethics of international research, in which the need to

take into account the reality of available and feasible health care is

a consideration of substantial importance.

In summary, these studies all address an urgent need in the countries

in which they are being conducted. They have been developed with

extensive in-country input and participation, and they are consistent

with widely accepted principles and guidelines of bioethics. Our

perspective and our decision to support these trials rest heavily on

local support and approval. In this regard, we point to the words of

Edward K. Mbidde, Chair, AIDS Research Committee, Uganda Cancer

Institute, in a letter, dated May 8, 1997, to the Director, NIH:

These are Ugandan studies conducted by Ugandan investigators

on Ugandans. [Elsewhere in the letter he discusses Ugandan

ethical review.] Due to lack of resources we have been

sponsored by organizations like yours [NIH]. We are grateful

that you have been able to do so.

There is a mix up of issues here which needs to be

clarified. It is not NIH conducting the studies in Uganda

but Ugandans conducting their study on their people for the

good of their people.

The issues surrounding these studies are, indeed, complex and subject

to some disagreement. However, final judgments about their

appropriateness must be heavily weighted in favor of decisions made at

the local level, as long as those decisions are consistent with

international standards and those of the U.S. We know of no other way

to realistically and rapidly address the gravity of mother-to-infant

transmission of HIV in the developing world and the current lack of a

proven, feasible intervention against it, than to continue the studies

on which our two agencies have embarked.

DATED: July 15, 1997

[Image] [Image]

Harold Varmus David Satcher

Director, National Institutes of Director, Centers for Disease Control

Health and Prevention

Appendix:

Recommendations From The Meeting On Mother-To-Infant

Transmission Of HIV By Use Of Antiretrovirals, Geneva 23-25 June 1994

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1A useful exploration of these events is contained in an article

written by Jon Cohen and published in Science, Vol. 269, pp. 624-626,

4 August 1995.