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 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
| l |
High-titer
|
|
|
| l |
Virus
can be concentrated
|
|
|
| l |
Transient
packaging due to VSV-G toxicity
|
|
|
|
l |
Adding
plasmids to 293T cells
|
|
|
|
l |
Plasmids
require SV40 T antigen expression
|
|
|
| l |
Variable
packaging and titers
|
|
|
| l |
Potential
recombinational events
|
|
|
| l |
Difficult
to scale-up as compared to stable lines
|