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| Stable
RD114 Packaging Line (M. |
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| Ward) |
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| l | Moloney
gag-pol in 3T3 cells |
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| l | Add
RD114 gene with phleomycin selection |
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| l | Isolate
high titer clones with NeoR gene and G418 |
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| l | Make
retroviral supernatants |
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| l | Concentrate
virus by centrifugation |
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| l | Can
transfer G418 resistance to human CD34+ |
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| cells |
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| l | Can
transfer normal globin gene into sickle |
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| CD34+
cells |
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