 |
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|
|
|
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|
|
|
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Precise coupling
between force and rMLC
|
|
phosphorylation
is quite variable and non-linear
|
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|
Maximal force can
be attained at ~ 0.2-0.3
|
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|
mol Pi/mol
rMLC.
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|
Phosphorylation
often declines during tension
|
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|
maintenance.
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Nonphosphorylated
myosin cross bridges
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|
contribute to
force generation
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Force generating
dephosphorylated cross bridges
|
|
|
could be
generated by dephosphorylating attached
|
|
cross bridges,
which are thought to have a slow
|
|
detachment rate
compared to phosphorylated
|
|
|
cross bridges or
by cooperative attachment of
|
|
dephosphorylated
cross bridges.
|
|
|
Cooperative
attachment is possibly regulated by
|
|
calponin and
caldesmon.
|
|