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| • |
MBS targets cGKIa to the SMC contractile
apparatus and activation of cGKIa
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increases PP1M
activity, the cGKIaincreases PP1M activity.
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| • |
Thromboxane
analog U46619 caused an increase in myosin light chain
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phosphorylation
from 10 to 68% in both vector and cGK1-59 transfected
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vascular smooth muscle
cells.
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| • |
In vector alone
transfected SMC, 8 Br-cGMP inhibited U46619 mediated
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myosin light
chain phosphorylation.
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| • |
Expression of
cGK1-59 diminished the ability of 8 Br-cGMP to inhibit myosin
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light chain
phosphorylation following U46619 stimulation.
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| • |
MBS assembles a
multienzyme complex tethering a phosphatase and at least
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two kinases (Rho,
cGK) with counter-regulatory effects.
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