Protein function and Enzyme kinetics

Proteins and Enzymes

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Properties of Amino Acids

Alaphatic amino acids
only carbon and hydrogen in side group

Aromatic Amino Acids

Amino acids with C-beta branching

Charged Amino Acids

Polar amino acids

Somewhat polar amino acids

Amino acids overlap in properties

How to think about amino acids

Tyrosine

Cysteine

Cystine andGlutathione

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The peptide bond

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Lock and key

Specific interactions at active site

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How do proteins function?

Actin and myosin: the contractile apparatus

Skeletal Muscle Cells

Skeletal Muscle Structure

Structure of Myosin

More myosin structure

Arrangement of Myosin Molecules in Thick Filaments

Myosin filament

Thin Filaments

Structure of actin filament

Actin in detail

Actin structure

Actin domains

Structure of a Sarcomere

Muscle Contraction

Stabilization of the Alignment of Thick and Thin Filaments

Crystal Structure of Myosin Head and Lever Arm

Regulation of Non-muscle Myosin II Assembly

Muscle continue

Muscle continue

Muscle continue

Muscle continue

Muscle continue

Muscle continue

Muscle continue

Muscle continue

Muscle continue

Myosin Superfamily

Three examples of the diverse structures of members of the myosin superfamily

In vitro Motility Assay

In vitro motility assay

Proteins as enzymes

Enzymes fall into classes based on function

Enzyme Kinetics

Enzyme reaction rates are determined by several factors.

Enzymes cont.

How we determine how fast an enzyme works

Mechaelis Menton kinetics

Plotting out our data it might look like this.

Lineweaver-Burke plot

Competitive inhibitors

Non-competitive inhibitor

Competitive/noncompetitive inhibitor

Effect of inhibitors