Now in the second step, attachment
2. En-bloc transfer of the
oligosaccharide to the
protein
One step transfer, catalyzed by
oligosaccharyl transferase, which
is bound to the membrane at the
translocator.
Covalently attached to certain
asparagines in the polypeptide
chain (said to be “N-linked”
glycosylation).
Attaches to NH2 side chain of
Asn but only in the context:
Asn-x-Ser  or   Asn-x-Thr