NER in E. coli continued
two single strand nicks are
placed to the 3' side of the
dimer (4 to 5 nucleotides)
and to the 5' side of the
dimer (8 nucleotides)
helicase II (uvrD) unwinds 12
- 13 b fragment containing
the dimer, ATP hydrolysis
required
DNA polymerase I fills in the
gap dNTPs required
DNA ligase seals the single
strand break ATP hydrolysis
to AMP required