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| • |
The
carboxy-terminus of Hp53 has been
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shown to play
an important role in
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controlling
the specific DNA binding
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function.
Wils type p53 is found in a latent
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form whicvh
does not bind to DNA. The
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specific DNA
binding activity was shown to
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be activated
by various pathways:
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phosphorylation
(Hupp et al., 1992), antibody
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specific for
the carboxy-terminus of the
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protein (Hupp
et al., 1992), small peptides
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which could
mimic the carboxy-terminus of
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the p53 (Hupp
et al., 1995), short single
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stranded DNA
(Jayaraman & Prives, 1995),
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deletion of
the last 30 amino-acids (Hupp et
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al., 1992)
and the interaction with a cellular
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protein
(Jayaraman et al., 1997).
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| • |
This
observation suggest that micro-
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injection of
antibodies such as PAb421
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induces an
activation of the transcriptional
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activity of
p53. Such hypothesis have been
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confirmed
(Hupp et al., 1995)
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