C2006/F2402 -'09 -- Corrections
Most Recent Corrections of lectures are Posted On Top.
Corrections to problem book are in order of book.
If you find any errors in outlines, recordings, problem book, or texts, please email Dr. M
(dbm2). Corrections to the lectures made after the lecture are marked
in blue
on the online version; significant
changes are listed
below.
Last Update: 05/10/2009 10:10 AM
Schedule
We are a bit behind the original schedule; topics
and readings have been adjusted to match what was actually covered. However the
topics, order, and readings per topic have not changed. Note that evening
lecture on April 14 will start at 5 pm NOT 5:40.
Live Lectures & Outlines
Lecture #10. Recent results indicate that hypersensitive sites may have loosened nucleosomes as well as (or instead of) TFs in place of histones. This has been added to lecture in several places. Also 'remodeling proteins' discussed in Sadava may not be a separate class of proteins, but TFs that turn on the modifying enzymes (resulting in remodeling). These changes are marked in blue.
Lecture #4, in the AM. I stated incorrectly that the adherens junction forming a band around the epithelial cells is closer to the apical surface that the band of tight junctions. It's the reverse, as the picture on handout 4A clearly shows.
Lecture #22. A few changes were made to the Lecture 22 notes after the
morning lecture. (A few problem numbers and subsection headings were wrong.)
Becker Text
Becker 6th & 7th ed., fig. 15-11. (c). Becker says human RBC have MT around their edges. According to the experts I consulted, mature human RBC do not contain MT. (They contain a spectrin web instead.) However RBC of other organism do have peripheral MT. Human platelets, but not human RBC, have MT.
Review Questions for Exam #1
The answer to 4B option (c) has an error. If the vesicles were formed by
invagination, the E side (not the P)
would be closest to the inside of the vesicle.
Problem Book
Corrections to Current 2009 Edition
Cover says "7th edition, revised." It should simply say "7th edition."
Problem 1-23 -- Answer. Complete explanation is not clear enough. It should read as follows. (Changes are in blue.)
Complete Explanation: This protein has only one hydrophobic section, which probably makes up a single transmembrane domain. That means that emerin is a single pass protein with 11 amino acids on one side of the membrane, a transmembrane section of 20-30 amino acids (approx.) and the rest of the protein (about 210 amino acids) on the other side of the membrane. Glycosylation happens only to amino acids in domains that are exposed to the lumen of the ER or Golgi; these are the domains that end up on the extracellular side of a plasma membrane protein. Phosphorylation happens inside the cell (in the cytoplasm, not just inside the ER or Golgi), where the kinases and phosphatases are. Since there is one possible glycosylation site and many possible phosphorylation sites, it makes the most sense if the short 11 amino acid tail is on the extracellular side and the large AA domain is intracellular.
The fact that the protein is found in the membrane fraction has nothing to do with the freeze fracture procedure. (See note in problem.)
Problem 2-6. In this problem, and in the answer, the term 'facilitated diffusion' is used to mean 'carrier mediated transport.' Transport through a channel is considered a separate case.
Problem 2-R F. There is a typo in one of the choices. The 5th choice should be (the region between TM 1 and TM 2) instead of (the region between TM 12 and TM 2).
Problem 3-13 -- Answer to Part E. Delete 'see picture for 3-2 part A'. There is no picture!
Problem 3-16. Consider the two plasma membrane proteins described in problem 1-20, not 1-21.
Problem 4-13, part A-1. It should refer to Question 11, not Question 10.
Problem 6-1. It should say 'Consider Protein P described in problem 1-17 (& 3-4)' not 1-18 (&3-2).
Problem 9R-3, explanation. The nicotinic cholinergic receptor is stimulated by nicotine, not inhibited by it.
Problem 9R-5. Part A-3. If cell has no alpha 1 receptors, NE should have no effect (unless it binds, although not as well as E, to beta 2 receptors). Question should say assume cell has both types of receptors.
Problem 12-10. Answer to part D. Explanation says 'Aldosterone affects primarily the proximal tubule...' Should say 'distal tubule' not proximal.
Problem 12-22. Answer to part E. What is labeled 'D-4' is the answer to E-1, and should be inserted before the other answers to part E. What are labeled 'E-1 to E-3' should be relabeled 'E-2 to E-4'.
Problem 12R-16. Answer to part B-3. The lower BP in the kidney will trigger a local response, which is the constriction of the efferent arteriole, or dilation of the afferent. (There is disagreement about which happens here.) The afferent only constricts in response to systemic input which overrides the local response in a case of a big drop in overall BP. (If there is a big drop in overall BP, for example because of sudden blood loss, the systemic input from the sympathetic system reduces GFR. This may temporarily impair kidney function, but is crucial to prevent water loss and restore blood volume.)