Microscopy, Cells, Organelles, Mitosis
For each laboratory session several electron micrographs are posted in the lab. Be sure that you examine these so that you understand the ultrastructural equivalents of the organelles and subcellular structures that you have seen under the microscope. There is a set of all electron micrographs used in the lab on reserve in the Health Sciences Library.
Learning objectives:
- Become familiar with the use of the light microscope (see p. 112 of this manual).
- Understand and be able to describe how the most common dye combination, hematoxylin and eosin (H&E) stains various components of cells and tissues.
- Identify cells and subcellular organelles.
- Relate the appearance of a cell as seen with the light microscope (LM) with that with the electron microscope (TEM, transmission electron microscope).
- Recognize and understand the stages in mitosis.
Lab Activities
- Cells, Organelles
1.1 Basic and Acid Stains
1.2 Histochemistry
1.3 Cellular Specializations
- Mitosis
- Electron Micrographs
- Questions
- Answers